ArticleGenes & development2012
Promoter-proximal polyadenylation sites reduce transcription activity.
Article in Genes & development, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- Transposable element-gene chimera cartography, origination and role in enhancing transcriptome plasticity.Nature structural & molecular biology · 2026Article
- Targeting Viral Transcription for HIV Cure Strategies.Microorganisms · 2024Review
- A CpG island-encoded mechanism protects genes from premature transcription termination.Nature communications · 2023Article
- Screening thousands of transcribed coding and non-coding regions reveals sequence determinants of RNA polymerase II elongation potential.Nature structural & molecular biology · 2022Article
- Expression of the miR-302/367 microRNA cluster is regulated by a conserved long non-coding host-gene.Scientific reports · 2021Article
- Accessible chromatin reveals regulatory mechanisms underlying cell fate decisions during early embryogenesis.Scientific reports · 2021Article
- Mapping domains of ARS2 critical for its RNA decay capacity.Nucleic acids research · 2020Article
- Transcription Terminator-Mediated Enhancement in Transgene Expression in Maize: Preponderance of the AUGAAU Motif Overlapping With Poly(A) Signals.Frontiers in plant science · 2020Article
- Chimeric 3' flanking regions strongly enhance gene expression in plants.Plant biotechnology journal · 2018Article
- Transcriptional Pause Sites Delineate Stable Nucleosome-Associated Premature Polyadenylation Suppressed by U1 snRNP.Molecular cell · 2018Article
- Experimental Verification and Evolutionary Origin of 5'-UTR Polyadenylation Sites inFrontiers in plant science · 2018Article
- Exploring the Impact of Cleavage and Polyadenylation Factors on Pre-mRNA Splicing Across Eukaryotes.G3 (Bethesda, Md.) · 2017Article
- mRNA-Associated Processes and Their Influence on Exon-Intron Structure in Drosophila melanogaster.G3 (Bethesda, Md.) · 2016Article
- DIS3 shapes the RNA polymerase II transcriptome in humans by degrading a variety of unwanted transcripts.Genome research · 2015Article
- Bidirectional Transcription Arises from Two Distinct Hubs of Transcription Factor Binding and Active Chromatin.Molecular cell · 2015Article
- The Importance of Controlling Transcription Elongation at Coding and Noncoding RNA Loci.Cold Spring Harbor symposia on quantitative biology · 2015Review
- Coupling mRNA processing with transcription in time and space.Nature reviews. Genetics · 2014Review
- Putting an 'End' to HIV mRNAs: capping and polyadenylation as potential therapeutic targets.AIDS research and therapy · 2013Article
- Article
- Competition within Introns: Splicing Wins over Polyadenylation via a General Mechanism.Acta naturae · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene expression relies on the functional communication between mRNA processing and transcription. We previously described the negative impact of a point-mutated splice donor (SD) site on transcription. Here we demonstrate that this mutation activates an upstream cryptic polyadenylation (CpA) site, which in turn causes reduced transcription. Functional depletion of U1 snRNP in the context of the wild-type SD triggers the same CpA event accompanied by decreased RNA levels. Thus, in accordance with recent findings, U1 snRNP can shield premature pA sites. The negative impact of unshielded pA sites on transcription requires promoter proximity, as demonstrated using artificial constructs and supported by a genome-wide data set. Importantly, transcription down-regulation can be recapitulated in a gene context devoid of splice sites by placing a functional bona fide pA site/transcription terminator within ~500 base pairs of the promoter. In contrast, promoter-proximal positioning of a pA site-independent histone gene terminator supports high transcription levels. We propose that optimal communication between a pA site-dependent gene terminator and its promoter critically depends on gene length and that short RNA polymerase II-transcribed genes use specialized termination mechanisms to maintain high transcription levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.