ArticleMolecular & cellular proteomics : MCP2013
A simple and effective cleavable linker for chemical proteomics applications.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- From Covalent Traps to Fluorescent Beacons: The Expanding Arsenal of Chemical Probes for Studying Ubiquitin and Ubiquitin-Like Proteins.Angewandte Chemie (International ed. in English) · 2026Review
- Targeting the Reactive Proteome: Recent Advances in Activity-Based Protein Profiling and Probe Design.Biomolecules · 2025Review
- Recent Advances in the Linkers of Drug Conjugates.Current medicinal chemistry · 2025Review
- Improved deconvolution of natural products' protein targets using diagnostic ions from chemical proteomics linkers.Beilstein journal of organic chemistry · 2024Review
- Synthesis, biological and molecular modelling for 1,3,4-thiadiazole sulfonyl thioureas: bacterial and fungal activity.RSC medicinal chemistry · 2023Article
- Thiourea derivatives containing 4-arylthiazoles and d-glucose moiety: design, synthesis, antimicrobial activity evaluation, and molecular docking/dynamics simulations.RSC medicinal chemistry · 2023Article
- Membrane protein channels equipped with a cleavable linker for inducing catalysis inside nanocompartments.Journal of materials chemistry. B · 2021Article
- Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries.Nature biotechnology · 2021Article
- Steric-Free Bioorthogonal Labeling of Acetylation Substrates Based on a Fluorine-Thiol Displacement Reaction.Journal of the American Chemical Society · 2021Article
- Secondary Amino Alcohols: Traceless Cleavable Linkers for Use in Affinity Capture and Release.Angewandte Chemie (International ed. in English) · 2020Article
- Activity-Based Protein Profiling Reveals That Cephalosporins Selectively Active on Non-replicatingFrontiers in microbiology · 2020Article
- Genetic encoding of 2-aryl-5-carboxytetrazole-based protein photo-cross-linkers.Chemical communications (Cambridge, England) · 2018Article
- Traceless Cleavage of Protein-Biotin Conjugates under Biologically Compatible Conditions.Chembiochem : a European journal of chemical biology · 2017Article
- Chalcone: A Privileged Structure in Medicinal Chemistry.Chemical reviews · 2017Review
- Activity-based probes for the ubiquitin conjugation-deconjugation machinery: new chemistries, new tools, and new insights.The FEBS journal · 2017Review
- Mapping the Binding Site of BMS-708163 on γ-Secretase with Cleavable Photoprobes.Cell chemical biology · 2017Article
- Chemical proteomics approaches for identifying the cellular targets of natural products.Natural product reports · 2016Review
- Cyclic acetals as cleavable linkers for affinity capture.Organic & biomolecular chemistry · 2015Article
- Comparative analysis of click chemistry mediated activity-based protein profiling in cell lysates.Molecules (Basel, Switzerland) · 2013Article
- Reversible protein affinity-labelling using bromomaleimide-based reagents.Organic & biomolecular chemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of metabolically labeled or probe-modified proteins is an important area in chemical proteomics. Isolation and purification of the protein targets is a necessary step before MS identification. The biotin-streptavidin system is widely used in this process, but the harsh denaturing conditions also release natively biotinylated proteins and non-selectively bound proteins. A cleavable linker strategy is a promising approach for solving this problem. Though several cleavable linkers have been developed and tested, an efficient, easily synthesized, and inexpensive cleavable linker is a desirable addition to the proteomics toolbox. Here, we describe the chemical proteomics application of a vicinal diol cleavable linker. Through easy-to-handle chemistry we incorporate this linker into an activity-based probe and a biotin alkyne tag amenable for bioorthogonal ligation. With these reagents, background protein identifications are significantly reduced relative to standard on-bead digestion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.