ArticleProceedings of the National Academy of Sciences of the United States of America2012
Bacillus cereus iron uptake protein fishes out an unstable ferric citrate trimer.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 33 citations in OpenAlex.
- Supramolecular Arrangement of Lignosulfonate-Based Iron Heteromolecular Complexes and Consequences of Their Interaction with CaJournal of agricultural and food chemistry · 2023Article
- The (Bio)Chemistry of Non-Transferrin-Bound Iron.Molecules (Basel, Switzerland) · 2022Review
- Siderophore for Lanthanide and Iron Uptake for Methylotrophy and Plant Growth Promotion inFrontiers in microbiology · 2022Article
- Hierarchical routing in carbon metabolism favors iron-scavenging strategy in iron-deficient soilProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Comparative Gut Microbiome Differences between Ferric Citrate and Calcium Carbonate Phosphate Binders in Patients with End-Stage Kidney Disease.Microorganisms · 2020Article
- Antiviral effects of ferric ammonium citrate.Cell discovery · 2018Article
- Structural investigations on orotate phosphoribosyltransferase from Mycobacterium tuberculosis, a key enzyme of the de novo pyrimidine biosynthesis.Scientific reports · 2017Article
- A genome-scale metabolic reconstruction of Lysinibacillus sphaericus unveils unexploited biotechnological potentials.PloS one · 2017Article
- Comparative Genomics of Iron-Transporting Systems in Bacillus cereus Strains and Impact of Iron Sources on Growth and Biofilm Formation.Frontiers in microbiology · 2016Article
- Coordination Chemistry of Microbial Iron Transport.Accounts of chemical research · 2015Article
- Direct evidence of iron uptake by the Gram-positive siderophore-shuttle mechanism without iron reduction.ACS chemical biology · 2014Article
- Iron acquisition in Bacillus cereus: the roles of IlsA and bacillibactin in exogenous ferritin iron mobilization.PLoS pathogens · 2014Article
- Ascorbate efflux as a new strategy for iron reduction and transport in plants.The Journal of biological chemistry · 2014Article
- Gram-positive siderophore-shuttle with iron-exchange from Fe-siderophore to apo-siderophore by Bacillus cereus YxeB.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Citrate is a common biomolecule that chelates Fe(III). Many bacteria and plants use ferric citrate to fulfill their nutritional requirement for iron. Only the Escherichia coli ferric citrate outer-membrane transport protein FecA has been characterized; little is known about other ferric citrate-binding proteins. Here we report a unique siderophore-binding protein from the gram-positive pathogenic bacterium Bacillus cereus that binds multinuclear ferric citrate complexes. We have demonstrated that B. cereus ATCC 14579 takes up (55)Fe radiolabeled ferric citrate and that a protein, BC_3466 [renamed FctC (ferric citrate-binding protein C)], binds ferric citrate. The dissociation constant (K(d)) of FctC at pH 7.4 with ferric citrate (molar ratio 1:50) is 2.6 nM. This is the tightest binding observed of any B. cereus siderophore-binding protein. Nano electrospray ionization-mass spectrometry (nano ESI-MS) analysis of FctC and ferric citrate complexes or citrate alone show that FctC binds diferric di-citrate, and triferric tricitrate, but does not bind ferric di-citrate, ferric monocitrate, or citrate alone. Significantly, the protein selectively binds triferric tricitrate even though this species is naturally present at very low equilibrium concentrations.
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