ArticleProceedings of the National Academy of Sciences of the United States of America2012
Using intein catalysis to probe the origin of major histocompatibility complex class I-presented peptides.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Unconventional modes of peptide-HLA-I presentation change the rules of TCR engagement.Discovery immunology · 2022Review
- Role for ribosome-associated quality control in sampling proteins for MHC class I-mediated antigen presentation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Presentation of Cryptic Peptides by MHC Class I Is Enhanced by Inflammatory Stimuli.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Nowhere to hide: unconventional translation yields cryptic peptides for immune surveillance.Immunological reviews · 2016Review
- Evaluating the Role of HLA-DM in MHC Class II-Peptide Association Reactions.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Ubiquitous Autofragmentation of Fluorescent Proteins Creates Abundant Defective Ribosomal Products (DRiPs) for Immunosurveillance.The Journal of biological chemistry · 2015Article
- Early Kinetics of the HLA Class I-Associated Peptidome of MVA.HIVconsv-Infected Cells.Journal of virology · 2015Article
- The nature and extent of contributions by defective ribosome products to the HLA peptidome.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Translating DRiPs: MHC class I immunosurveillance of pathogens and tumors.Journal of leukocyte biology · 2014Review
- Re-examining class-I presentation and the DRiP hypothesis.Trends in immunology · 2014Review
- Article
- Monitoring peptide processing for MHC class I molecules in the endoplasmic reticulum.Current opinion in immunology · 2014Review
- Substrate-induced protein stabilization reveals a predominant contribution from mature proteins to peptides presented on MHC class I.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Kinetics of antigen expression and epitope presentation during virus infection.PLoS pathogens · 2013Article
- Premature translational termination products are rapidly degraded substrates for MHC class I presentation.PloS one · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
All vertebrate nucleated cells generate peptides from their expressed gene products and then display them at the cell surface bound to MHC class I molecules. This allows CD8(+) T cells to detect and eliminate abnormal cells that are synthesizing foreign proteins, e.g., from viruses or mutations. To permit the immune system to more uniformly monitor a cell's proteins, regardless of their half-life or location, it has been thought that the products of rapid degradation of the mistakes of protein synthesis (defective ribosomal products, DRiPs) preferentially contribute to the class I-presented peptides. However, using intein catalysis to generate peptide sequences exclusively by posttranslational splicing of mature proteins, we show here that presented peptides can be generated from fully folded and functional proteins. Remarkably, the presentation of peptides from two model mature proteins is just as efficient as from newly synthesized proteins subject to errors in translation or folding. These results indicate that for the constructs we have analyzed, DRiPs are not a more efficient source of class I peptides for antigen presentation than the turnover of mature functional proteins. Accordingly, our data suggest that one of the major ways the immune system evaluates the health of cells is by monitoring the breakdown products of the proteome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.