ArticleProceedings of the National Academy of Sciences of the United States of America2012
Targeted microRNA expression in dairy cattle directs production of β-lactoglobulin-free, high-casein milk.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 97 citations in OpenAlex.
- Bibliometric Analysis of Milk Protein Gene Expression Regulation Using CiteSpace and VOSviewer.Molecular biotechnology · 2026Review
- Investigating Dietary microRNA Stability and Function Using a Transgenic Milk Model With Unique microRNA Sequences.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Outlook on genome editing application to cattle.Journal of veterinary science · 2024Review
- miR-380-3p promotes β-casein expression by targeting αS1-casein in goat mammary epithelial cells.Animal bioscience · 2023Article
- Emerging Roles of Noncoding RNAs in Bovine Mastitis Diseases.Pathogens (Basel, Switzerland) · 2022Review
- Physiological impact of the environment on the welfare of transgenic goats raised in a tropical climate.International journal of biometeorology · 2021Article
- Holstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change.BMC genomics · 2021Article
- 25th ANNIVERSARY OF CLONING BY SOMATIC-CELL NUCLEAR TRANSFER: Nuclear transfer and the development of genetically modified/gene edited livestock.Reproduction (Cambridge, England) · 2021Review
- β-Lactoglobulin and Glycodelin: Two Sides of the Same Coin?Frontiers in physiology · 2021Review
- Harnessing genomic information for livestock improvement.Nature reviews. Genetics · 2019Review
- Global mapping of binding sites for phic31 integrase in transgenic maden-darby bovine kidney cells using ChIP-seq.Hereditas · 2019Article
- Proposed U.S. regulation of gene-edited food animals is not fit for purpose.NPJ science of food · 2019Review
- Production of hypoallergenic milk from DNA-free beta-lactoglobulin (BLG) gene knockout cow using zinc-finger nucleases mRNA.Scientific reports · 2018Article
- Article
- Cattle with a precise, zygote-mediated deletion safely eliminate the major milk allergen beta-lactoglobulin.Scientific reports · 2018Article
- Development of genome engineering technologies in cattle: from random to specific.Journal of animal science and biotechnology · 2018Review
- Taillessness in a Cloned Cow is Not Genetically Transmitted.Cellular reprogramming · 2017Article
- Human lactoferrin efficiently targeted into caprine beta-lactoglobulin locus with transcription activator-like effector nucleases.Asian-Australasian journal of animal sciences · 2017Article
- Genome editing and genetic engineering in livestock for advancing agricultural and biomedical applications.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- miR-148a and miR-17-5p synergistically regulate milk TAG synthesis via PPARGC1A and PPARA in goat mammary epithelial cells.RNA biology · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Milk from dairy cows contains the protein β-lactoglobulin (BLG), which is not present in human milk. As it is a major milk allergen, we wished to decrease BLG levels in milk by RNAi. In vitro screening of 10 microRNAs (miRNAs), either individually or in tandem combinations, identified several that achieved as much as a 98% knockdown of BLG. One tandem construct was expressed in the mammary gland of an ovine BLG-expressing mouse model, resulting in 96% knockdown of ovine BLG in milk. Following this in vivo validation, we produced a transgenic calf, engineered to express these tandem miRNAs. Analysis of hormonally induced milk from this calf demonstrated absence of BLG and a concurrent increase of all casein milk proteins. The findings demonstrate miRNA-mediated depletion of an allergenic milk protein in cattle and validate targeted miRNA expression as an effective strategy to alter milk composition and other livestock traits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.