ArticleProceedings of the National Academy of Sciences of the United States of America2012
XAX1 from glycosyltransferase family 61 mediates xylosyltransfer to rice xylan.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
88 citing papers in PubMed, 210 citations in OpenAlex.
- Multiplexed Engineering of Disease Resistance and Lodging Tolerance in Wheat Via a Single Xylan Modifying Glycosyltransferase.Plant biotechnology journal · 2026Article
- Targeted multiplex gene knockouts in Lemna minor using CRISPR/Cas9.Transgenic research · 2026Article
- Genomic and transcriptomic basis of morphological and life cycle diversity in the prasinophyte alga Pseudoscourfieldia marina.Communications biology · 2026Article
- Crystal structure of epidermal growth factor domain-specificPNAS nexus · 2026Article
- Fine-tuning xylan side chains for enhanced production of high-yield sugars, fine-shaped nanocrystals, and stable Pickering emulsions in Arabidopsis thaliana.Biotechnology for biofuels and bioproducts · 2026Article
- Enhancing maize resistance to Fusarium verticillioides through modulation of cell wall structure and components by ZmXYXT2.Journal of advanced research · 2025Article
- Cell wall-related glycosyltransferases and wall architecture in the model liverwort Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2025Article
- Lignocellulose adaptation drives polysaccharide biosynthesis inFrontiers in fungal biology · 2025Article
- XYLAN O-ACETYLTRANSFERASE 6 promotes xylan synthesis by forming a complex with IRX10 and governs wall formation in rice.The Plant cell · 2024Article
- Identification of glycosyltransferases mediating 2-O-arabinopyranosyl and 2-O-galactosyl substitutions of glucuronosyl side chains of xylan.The Plant journal : for cell and molecular biology · 2024Article
- A sucrose ferulate cycle linchpin for ferulyolation of arabinoxylans in plant commelinids.Nature plants · 2024Article
- Article
- Silencing ScGUX2 reduces xylan glucuronidation and improves biomass saccharification in sugarcane.Plant biotechnology journal · 2024Article
- A comprehensive review on genetic modification of plant cell wall for improved saccharification efficiency.Molecular biology reports · 2023Review
- Lignin, the Lignification Process, and Advanced, Lignin-Based Materials.International journal of molecular sciences · 2023Review
- NAC transcription factors ATAF1 and ANAC055 affect the heat stress response in Arabidopsis.Scientific reports · 2022Article
- Modification of plant cell walls with hydroxycinnamic acids by BAHD acyltransferases.Frontiers in plant science · 2022Review
- Characteristics and candidate genes associated with excellent stalk strength in maize (Frontiers in plant science · 2022Article
- CRISPR/Cas9 suppression of OsAT10, a rice BAHD acyltransferase, reduces p-coumaric acid incorporation into arabinoxylan without increasing saccharification.Frontiers in plant science · 2022Article
- Functional analysis of GT61 glycosyltransferases from grass species in xylan substitutions.Planta · 2021Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 7 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xylan is the second most abundant polysaccharide on Earth and represents an immense quantity of stored energy for biofuel production. Despite its importance, most of the enzymes that synthesize xylan have yet to be identified. Xylans have a backbone of β-1,4-linked xylose residues with substitutions that include α-(1→2)-linked glucuronosyl, 4-O-methyl glucuronosyl, and α-1,2- and α-1,3-arabinofuranosyl residues. The substitutions are structurally diverse and vary by taxonomy, with grass xylan representing a unique composition distinct from dicots and other monocots. To date, no enzyme has yet been identified that is specific to grass xylan synthesis. We identified a xylose-deficient loss-of-function rice mutant in Os02g22380, a putative glycosyltransferase in a grass-specific subfamily of family GT61. We designate the mutant xax1 for xylosyl arabinosyl substitution of xylan 1. Enzymatic fingerprinting of xylan showed the specific absence in the mutant of a peak, which was isolated and determined by (1)H-NMR to be (β-1,4-Xyl)(4) with a β-Xylp-(1→2)-α-Araf-(1→3). Rice xax1 mutant plants are deficient in ferulic and coumaric acid, aromatic compounds known to be attached to arabinosyl residues in xylan substituted with xylosyl residues. The xax1 mutant plants exhibit an increased extractability of xylan and increased saccharification, probably reflecting a lower degree of diferulic cross-links. Activity assays with microsomes isolated from tobacco plants transiently expressing XAX1 demonstrated xylosyltransferase activity onto endogenous acceptors. Our results provide insight into grass xylan synthesis and how substitutions may be modified for increased saccharification for biofuel generation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.