ArticleProceedings of the National Academy of Sciences of the United States of America2012
Alphaherpesvirus axon-to-cell spread involves limited virion transmission.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 93 citations in OpenAlex.
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- Transneuronal Circuit Analysis with Pseudorabies Viruses.Current protocols · 2023Review
- A Single Herpes Simplex Virus 1 Genome Reactivates from Individual Cells.Microbiology spectrum · 2022Article
- Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Mutational pressure by host APOBEC3s more strongly affects genes expressed early in the lytic phase of herpes simplex virus-1 (HSV-1) and human polyomavirus (HPyV) infection.PLoS pathogens · 2021Article
- Viral infection of human neurons triggers strain-specific differences in host neuronal and viral transcriptomes.PLoS pathogens · 2021Article
- Mapping of spinal interneurons involved in regulation of the lower urinary tract in juvenile male rats.IBRO reports · 2020Article
- Biographical Feature: The Long and Winding Road: My Career Path and How I Ended Up Where I Am Now.Journal of virology · 2020Article
- HSV-1 Cytoplasmic Envelopment and Egress.International journal of molecular sciences · 2020Review
- Abortive herpes simplex virus infection of nonneuronal cells results in quiescent viral genomes that can reactivate.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- 3D Printed Neural Regeneration Devices.Advanced functional materials · 2020Article
- Viral cell-to-cell spread: Conventional and non-conventional ways.Advances in virus research · 2020Review
- Microtubule-Dependent Trafficking of Alphaherpesviruses in the Nervous System: The Ins and Outs.Viruses · 2019Review
- Hitchhiking on the neuronal highway: Mechanisms of transsynaptic specificity.Journal of chemical neuroanatomy · 2019Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The spread of viral infection within a host can be restricted by bottlenecks that limit the size and diversity of the viral population. An essential process for alphaherpesvirus infection is spread from axons of peripheral nervous system neurons to cells in peripheral epithelia (anterograde-directed spread, ADS). ADS is necessary for the formation of vesicular lesions characteristic of reactivated herpesvirus infections; however, the number of virions transmitted is unknown. We have developed two methods to quantitate ADS events using a compartmentalized neuronal culture system. The first method uses HSV-1 and pseudorabies virus recombinants that express one of three different fluorescent proteins. The fluorescence profiles of cells infected with the virus mixtures are used to quantify the number of expressed viral genomes. Strikingly, although epithelial or neuronal cells express 3-10 viral genomes after infection by free virions, epithelial cells infected by HSV-1 or pseudorabies virus following ADS express fewer than two viral genomes. The second method uses live-cell fluorescence microscopy to track individual capsids involved in ADS. We observed that most ADS events involve a single capsid infecting a target epithelial cell. Together, these complementary analyses reveal that ADS events are restricted to small numbers of viral particles, most often a single virion, resulting in a single viral genome initiating infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.