ArticleMethods in molecular biology (Clifton, N.J.)2013
Mica functionalization for imaging of DNA and protein-DNA complexes with atomic force microscopy.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 118 citations in OpenAlex.
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- Toehold-Mediated Shape Transition of Nucleic Acid Nanoparticles.ACS applied materials & interfaces · 2023Article
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- Biophysical, Molecular and Proteomic Profiling of Human Retinal Organoid-Derived Exosomes.Pharmaceutical research · 2023Article
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- Predicting the placement of biomolecular structures on AFM substrates based on electrostatic interactions.Frontiers in molecular biosciences · 2023Article
- Characterization of RNA Nanoparticles and Their Dynamic Properties Using Atomic Force Microscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Concentration and time-dependent amyloidogenic characteristics of intrinsically disordered N-terminal region ofFrontiers in microbiology · 2023Article
- Structural and dynamic basis of DNA capture and translocation by mitochondrial Twinkle helicase.Nucleic acids research · 2022Article
- Modular nanoarray vaccine for SARS-CoV-2.Nanomedicine : nanotechnology, biology, and medicine · 2022Article
- Nanorings to Probe Mechanical Stress of Single-Stranded DNA Mediated by the DNA Duplex.International journal of molecular sciences · 2022Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Surface preparation is a key step for reliable and reproducible imaging of DNA and protein-DNA complexes with atomic force microscopy (AFM). This article describes the approaches for chemical functionalization of the mica surface. One approach utilizes 3-aminopropyl-trietoxy silane (APTES), enabling one to obtain a smooth surface termed AP-mica. This surface binds nucleic acids and nucleoprotein complexes in a wide range of ionic strengths, in the absence of divalent cations and in a broad range of pH. Another method utilizes aminopropyl silatrane (APS) to yield an APS-mica surface. The advantage of APS-mica compared with AP-mica is the ability to obtain reliable and reproducible time-lapse images in aqueous solutions. The chapter describes the methodologies for the preparation of AP-mica and APS-mica surfaces and the preparation of samples for AFM imaging. The protocol for synthesis and purification of APS is also provided. The applications are illustrated with a number of examples.
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