ArticleBiomedical optics express2012
Three-color femtosecond source for simultaneous excitation of three fluorescent proteins in two-photon fluorescence microscopy.
Article in Biomedical optics express, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Simultaneous label-free autofluorescence multi-harmonic microscopy driven by the supercontinuum generated from a bulk nonlinear crystal.Biomedical optics express · 2024Article
- Two-Photon Imaging for Non-Invasive Corneal Examination.Sensors (Basel, Switzerland) · 2022Review
- Image-seq: spatially resolved single-cell sequencing guided by in situ and in vivo imaging.Nature methods · 2022Article
- Femtosecond Er-doped fiber laser source tunable from 872 to 1075 nm for two-photon vision studies in humans.Biomedical optics express · 2022Article
- Single-laser-based simultaneous four-wavelength excitation source for femtosecond two-photon fluorescence microscopy.Biomedical optics express · 2021Article
- Low noise, self-phase-modulation-enabled femtosecond fiber sources tunable in 740-1236 nm for wide two-photon fluorescence microscopy applications.Biomedical optics express · 2021Article
- Synchronously pumped Raman laser for simultaneous degenerate and nondegenerate two-photon microscopy.Biomedical optics express · 2021Article
- Serial imaging of micro-agents and cancer cell spheroids in a microfluidic channel using multicolor fluorescence microscopy.PloS one · 2021Article
- Label-free, High-Resolution Optical Metabolic Imaging of Human Cervical Precancers Reveals Potential for Intraepithelial Neoplasia Diagnosis.Cell reports. Medicine · 2020Article
- Single-cavity dual-wavelength all-fiber femtosecond laser for multimodal multiphoton microscopy.Biomedical optics express · 2020Article
- Frontiers in Intravital Multiphoton Microscopy of Cancer.Cancer reports (Hoboken, N.J.) · 2020Review
- Miniaturized multimodal multiphoton microscope for simultaneous two-photon and three-photon imaging with a dual-wavelength Er-doped fiber laser.Biomedical optics express · 2020Article
- SyncRGB-FLIM: synchronous fluorescence imaging of red, green and blue dyes enabled by ultra-broadband few-cycle laser excitation and fluorescence lifetime detection.Biomedical optics express · 2019Article
- Custom fabrication and mode-locked operation of a femtosecond fiber laser for multiphoton microscopy.Scientific reports · 2019Article
- Investigation of the long wavelength limit of soliton self-frequency shift in a silica fiber.Optics express · 2018Article
- Divided pulse soliton self-frequency shift: a multi-color, dual-polarization, power-scalable, broadly tunable optical source.Optics letters · 2017Article
- Characterization and adaptive compression of a multi-soliton laser source.Optics express · 2017Article
- Simple approach to three-color two-photon microscopy by a fiber-optic wavelength convertor.Biomedical optics express · 2016Article
- Femtosecond laser bone ablation with a high repetition rate fiber laser source.Biomedical optics express · 2015Article
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Authors and funding
6 authors.
Funding
Abstract
We demonstrate a fiber-based, three-color femtosecond source for simultaneous imaging of three fluorescent proteins (FPs) using two-photon fluorescence microscopy (2PM). The three excitation wavelengths at 775 nm, 864 nm and 950 nm, are obtained through second harmonic generation (SHG) of the 1550-nm pump laser and the 1728-nm and 1900-nm solitons generated through soliton self-frequency shift (SSFS) in a large-mode-area (LMA) fiber. These energetic pulses are well matched to the two-photon excitation peaks of red, cyan and yellow fluorescent proteins (TagRFPs, TagCFPs, and TagYFPs) for efficient excitation. We demonstrate simultaneous 2PM of human melanoma cells expressing a "rainbow" combination of these three fluorescent proteins.
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