ArticleInfectious diseases in obstetrics and gynecology2012
Susceptibility of Gardnerella vaginalis biofilms to natural antimicrobials subtilosin, ε-poly-L-lysine, and lauramide arginine ethyl ester.
Article in Infectious diseases in obstetrics and gynecology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
- Antibiofilm Agents for the Treatment and Prevention of Bacterial Vaginosis: A Systematic Narrative Review.The Journal of infectious diseases · 2024Pooled it
- Investigating the antimicrobial and antibiofilm properties of marine halophilic Bacillus species against ESKAPE pathogens.Environmental microbiology reports · 2024Article
- Interpretation of vaginal metagenomic characteristics in different types of vaginitis.mSystems · 2024Article
- Identification and characterization of novel endolysins targeting Gardnerella vaginalis biofilms to treat bacterial vaginosis.NPJ biofilms and microbiomes · 2022Article
- AntimicrobialAntibiotics (Basel, Switzerland) · 2022Review
- The Female Vaginal Microbiome in Health and Bacterial Vaginosis.Frontiers in cellular and infection microbiology · 2021Review
- Crystal Violet Staining Alone Is Not Adequate to Assess Synergism or Antagonism in Multi-Species Biofilms of Bacteria Associated With Bacterial Vaginosis.Frontiers in cellular and infection microbiology · 2021Article
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- Article
- Potential Probiotics Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895 Co-Aggregate with Clinical Isolates of Proteus mirabilis and Prevent Biofilm Formation.Probiotics and antimicrobial proteins · 2020Article
- The Battle of Probiotics and Their Derivatives Against Biofilms.Infection and drug resistance · 2020Review
- Article
- Antimicrobial Susceptibility Testing of Metronidazole and Clindamycin againstThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2020Article
- Comparative Evaluation of Combinatory Interaction between Endocannabinoid System Compounds and Poly-L-lysine againstBioMed research international · 2020Article
- Cationic amphiphiles against Gardnerella vaginalis resistant strains and bacterial vaginosis-associated pathogens.Pathogens and disease · 2019Article
- Cigarette smoking is associated with an altered vaginal tract metabolomic profile.Scientific reports · 2018Article
- Fighting biofilms with lantibiotics and other groups of bacteriocins.NPJ biofilms and microbiomes · 2018Review
- Gemini Cationic Amphiphiles Control Biofilm Formation by Bacterial Vaginosis Pathogens.Antimicrobial agents and chemotherapy · 2017Article
- Elucidation of Bacillus subtilis KATMIRA 1933 Potential for Spore Production in Submerged Fermentation of Plant Raw Materials.Probiotics and antimicrobial proteins · 2017Article
- Natural-Product Antibiotics: Cues for Modulating Bacterial Biofilm Formation.Trends in microbiology · 2017Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Bacterial vaginosis is a common vaginal infection associated with numerous gynecological and obstetric complications. This condition is characterized by the presence of thick adherent vaginal biofilms, composed mainly of Gardnerella vaginalis. This organism is thought to be the primary aetiological cause of the infection paving the way for various opportunists to colonize the niche. Previously, we reported that the natural antimicrobials subtilosin, ε-poly-L-lysine, and lauramide arginine ethyl ester selectively inhibit the growth of this pathogen. In this study, we used plate counts to evaluate the efficacy of these antimicrobials against established biofilms of G. vaginalis. Additionally, we validated and compared two rapid methods (ATP viability and resazurin assays) for the assessment of cell viability in the antimicrobial-treated G. vaginalis biofilms. Out of the tested antimicrobials, lauramide arginine ethyl ester had the strongest bactericidal effect, followed by subtilosin, with clindamycin and polylysine showing the weakest effect. In comparison to plate counts, ATP viability and resazurin assays considerably underestimated the bactericidal effect of some antimicrobials. Our results indicate that these assays should be validated for every new application.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.