ArticleJournal of bacteriology2012
Two transporters essential for reassimilation of novel cholate metabolites by Rhodococcus jostii RHA1.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Metabolism of bile salts in the estrogen degrading bacterium Caenibius tardaugens.Biodegradation · 2026Article
- Degradation of CCurrent microbiology · 2025Article
- Insight into Different Stages of Steroid Degradation in ThermophilicInternational journal of molecular sciences · 2022Article
- The unusual convergence of steroid catabolic pathways inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Bacterial Hydratases Involved in Steroid Side Chain Degradation Have Distinct Substrate Specificities.Journal of bacteriology · 2022Article
- Comparative Analysis of Bile-Salt Degradation inApplied and environmental microbiology · 2021Article
- Investigations on the Degradation of the Bile Salt Cholate via the 9,10-Microorganisms · 2021Article
- Proteome, Bioinformatic, and Functional Analyses Reveal a Distinct and Conserved Metabolic Pathway for Bile Salt Degradation in theApplied and environmental microbiology · 2021Article
- Degradation of Bile Acids by Soil and Water Bacteria.Microorganisms · 2021Review
- Substrate Inhibition of 5β-ΔFrontiers in microbiology · 2021Article
- Steroids originating from bacterial bile acid degradation affect Caenorhabditis elegans and indicate potential risks for the fauna of manured soils.Scientific reports · 2019Article
- Review
- New insights into the genome of Rhodococcus ruber strain Chol-4.BMC genomics · 2019Article
- Genome Sequence of the Bile Salt-Degrading BacteriumGenome announcements · 2018Article
- A Rhodococcal Transcriptional Regulatory Mechanism Detects the Common Lactone Ring of AHL Quorum-Sensing Signals and Triggers the Quorum-Quenching Response.Frontiers in microbiology · 2018Article
- A Novel Steroid-Coenzyme A Ligase from Novosphingobium sp. Strain Chol11 Is Essential for an Alternative Degradation Pathway for Bile Salts.Applied and environmental microbiology · 2018Article
- Article
- Characterization of novel acyl coenzyme A dehydrogenases involved in bacterial steroid degradation.Journal of bacteriology · 2015Article
- Substrate uptake and subcellular compartmentation of anoxic cholesterol catabolism in Sterolibacterium denitrificans.The Journal of biological chemistry · 2015Article
- Substrate specificities and conformational flexibility of 3-ketosteroid 9α-hydroxylases.The Journal of biological chemistry · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The bacterial uptake of steroids and their metabolites remains poorly understood. We investigated two transporters associated with cholate catabolism in Rhodococcus jostii RHA1. Reverse transcriptase quantitative-PCR indicated that an ATP-binding cassette (ABC) transporter and a major facilitator superfamily (MFS) transporter were upregulated 16.7- and 174-fold, respectively, during the exponential phase of growth on cholate compared to growth on pyruvate. Gene knockout analysis established that these transporters are required for the reassimilation of distinct metabolites that accumulate during growth on cholate. The ABC transporter, encoded by camABCD, was essential for uptake of 1β(2'-propanoate)-3aα-H-4α(3"(R)-hydroxy-3"-propanoate)-7aβ-methylhexahydro-5-indanone and a desaturated analog. The MFS transporter, encoded by camM, was essential for uptake of 3,7(R),12(S)-trihydroxy-9-oxo-9,10-seco-23,24-bisnorchola-1,3,5(10)-trien-22-oate. These metabolites differ from cholate metabolites reported to be excreted by proteobacteria in that they retain an isopropanoyl side chain at C-17. The uptake of these metabolites was necessary for maximal growth on cholate: a ΔcamB mutant lacking the permease component of the ABC transporter and a ΔcamM mutant lacking the MFS transporter grew to 74% and 77%, respectively, of the yield of the wild type. This study demonstrates for the first time the requirement for specific transporters for uptake of cholate metabolites and highlights the importance and complexity of transport processes associated with bacterial steroid catabolism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.