ArticleNucleic acids research2012
A multipronged strategy of an anti-terminator protein to overcome Rho-dependent transcription termination.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- The Psu protein of phage satellite P4 inhibits transcription termination factor ρ by forced hyper-oligomerization.Nature communications · 2025Article
- A Screen formBio · 2017Article
- Transcription is regulated by NusA:NusG interaction.Nucleic acids research · 2016Article
- Transcription Elongation Factor NusA Is a General Antagonist of Rho-dependent Termination in Escherichia coli.The Journal of biological chemistry · 2016Article
- HK022 Nun Requires Arginine-Rich Motif Residues Distinct from λ N.Journal of bacteriology · 2015Article
- N protein from lambdoid phages transforms NusA into an antiterminator by modulating NusA-RNA polymerase flap domain interactions.Nucleic acids research · 2015Article
- Bacteriophage lambda: Early pioneer and still relevant.Virology · 2015Review
- Distinct pathways of RNA polymerase regulation by a phage-encoded factor.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Redundancy of primary RNA-binding functions of the bacterial transcription terminator Rho.Nucleic acids research · 2014Article
- The interaction surface of a bacterial transcription elongation factor required for complex formation with an antiterminator during transcription antitermination.The Journal of biological chemistry · 2013Article
- Host adaption to the bacteriophage carrier state of Campylobacter jejuni.Research in microbiologyArticle
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the important role of Rho-dependent transcription termination in bacteria is to prevent gene expressions from the bacteriophage DNA. The transcription anti-termination systems of the lambdoid phages have been designed to overcome this Rho action. The anti-terminator protein N has three interacting regions, which interact with the mRNA, with the NusA and with the RNA polymerase. Here, we show that N uses all these interaction modules to overcome the Rho action. N and Rho co-occupy their overlapping binding sites on the nascent RNA (the nutR/tR1 site), and this configuration slows down the rate of ATP hydrolysis and the rate of RNA release by Rho from the elongation complex. N-RNA polymerase interaction is not too important for this Rho inactivation process near/at the nutR site. This interaction becomes essential when the elongation complex moves away from the nutR site. From the unusual NusA-dependence property of a Rho mutant E134K, a suppressor of N, we deduced that the N-NusA complex in the anti-termination machinery reduces the efficiency of Rho by removing NusA from the termination pathway. We propose that NusA-remodelling is also one of the mechanisms used by N to overcome the termination signals.
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