ArticleMolecular plant2013
AtPRK2 promotes ROP1 activation via RopGEFs in the control of polarized pollen tube growth.
Article in Molecular plant, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
79 citing papers in PubMed, 161 citations in OpenAlex.
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Rab5 GTPases mediate the targeting of ROP signalling to establish polarity for pollen germination.Nature plants · 2025Article
- A GhSnRK10-GhRopGEF5-GhROP10 signaling pathway regulates cotton fiber cell growth.Plant communications · 2025Article
- RLCKs phosphorylate RopGEFs to control auxin-dependent Arabidopsis development.Nature plants · 2025Article
- OsPRK1/2/3-mediated reactive oxygen species signaling is required for pollen tube germination in rice.Journal of integrative plant biology · 2025Article
- Polarized subcellular activation of Rho proteins by specific ROPGEFs drives pollen germination in Arabidopsis thaliana.PLoS biology · 2025Article
- Transcriptome profiling of foxtail millet (Setaria italica) pollen and anther.BMC plant biology · 2024Article
- Arabidopsis class A S-acyl transferases modify the pollen receptors LIP1 and PRK1 to regulate pollen tube guidance.The Plant cell · 2024Article
- Characterization, evolution, and abiotic stress responses of leucine-rich repeat receptor-like protein kinases (LRR-RLK) in Liriodendron chinense.BMC genomics · 2024Article
- Heat stress at the bicellular stage inhibits sperm cell development and transport into pollen tubes.Plant physiology · 2024Article
- RHO OF PLANTS signalling and the activating ROP GUANINE NUCLEOTIDE EXCHANGE FACTORS: specificity in cellular signal transduction in plants.Journal of experimental botany · 2024Review
- TheInternational journal of molecular sciences · 2024Article
- A large-scale screening identifies receptor-like kinases with common features in kinase domains that are potentially related to disease resistance in planta.Frontiers in plant science · 2024Article
- PcLRR-RK3, an LRR receptor kinase is required for growth andMycology · 2024Article
- VAMP726 and VAMP725 regulate vesicle secretion and pollen tube growth in Arabidopsis.Plant cell reports · 2023Article
- PXL1 and SERKs act as receptor-coreceptor complexes for the CLE19 peptide to regulate pollen development.Nature communications · 2023Article
- Signaling network controlling ROP-mediated tip growth in Arabidopsis and beyond.Plant communications · 2023Review
- Barley guanine nucleotide exchange factor HvGEF14 is an activator of the susceptibility factor HvRACB and supports host cell entry by Blumeria graminis f. sp. hordei.Molecular plant pathology · 2022Article
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
- A small Rho GTPase OsRacB is required for pollen germination in rice.Development, growth & differentiation · 2022Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The ROP1 GTPase-based signaling network controls tip growth in Arabidopsis pollen tubes. Our previous studies imply that ROP1 might be directly activated by RopGEF1, which belongs to a plant-specific family of Rho guanine nucleotide exchange factors (RopGEFs) and in turn may be activated by an unknown factor through releasing RopGEF1's auto-inhibition. In this study, we found that RopGEF1 forms a complex with ROP1 and AtPRK2, a receptor-like protein kinase previously shown to interact with RopGEFs. AtPRK2 phosphorylated RopGEF1 in vitro and the atprk1,2,5 triple mutant showed defective pollen tube growth, similar to the phenotype of the ropgef1,9,12,14 quadruple mutant. Overexpression of a dominant negative form of AtPRK2 (DN-PRK2) inhibited pollen germination in Arabidopsis and reduced pollen elongation in tobacco. The DN-PRK2-induced pollen germination defect was rescued by overexpressing a constitutively active form of RopGEF1, RopGEF1(90-457), implying that RopGEF1 acts downstream of AtPRK2. Moreover, AtPRK2 increased ROP1 activity at the apical plasma membrane whereas DN-PRK2 reduced ROP1 activity. Finally, two mutations at the C-terminal putative phosphorylation sites of RopGEF1 (RopGEF1S460A and RopGEF1S480A) eliminated the function of RopGEF1 in vivo. Taken together, our results support the hypothesis that AtPRK2 acts as a positive regulator of the ROP1 signaling pathway most likely by activating RopGEF1 through phosphorylation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.