ArticleApplied and environmental microbiology2012
A Single-cell genome for Thiovulum sp.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- Microbial respiration - a biomineral perspective.FEMS microbiology ecology · 2025Review
- Synergistic phenotypic adaptations of motile purple sulphur bacteria Chromatium okenii during lake-to-laboratory domestication.PloS one · 2024Article
- Fluorescence Microscopy Study of the Intracellular Sulfur Globule Protein SgpD in the Purple Sulfur BacteriumMicroorganisms · 2023Article
- Cave Thiovulum (Candidatus Thiovulum stygium) differs metabolically and genomically from marine species.The ISME journal · 2023Article
- Insights into the dynamics between viruses and their hosts in a hot spring microbial mat.The ISME journal · 2020Article
- The CURE for Cultivating Fastidious Microbes.Journal of microbiology & biology education · 2019Article
- Single-cell metagenomics: challenges and applications.Protein & cell · 2018Review
- Obtaining high-quality draft genomes from uncultured microbes by cleaning and co-assembly of single-cell amplified genomes.Scientific reports · 2018Article
- Genomic Insight Into the Predominance of Candidate Phylum Atribacteria JS1 Lineage in Marine Sediments.Frontiers in microbiology · 2018Article
- Optofluidic Single-Cell Genome Amplification of Sub-micron Bacteria in the Ocean Subsurface.Frontiers in microbiology · 2018Article
- The trajectory of microbial single-cell sequencing.Nature methods · 2017Review
- Mixed transmission modes and dynamic genome evolution in an obligate animal-bacterial symbiosis.The ISME journal · 2017Article
- Tools for Genomic and Transcriptomic Analysis of Microbes at Single-Cell Level.Frontiers in microbiology · 2017Review
- Comparative Genomic Analysis of the ClassFrontiers in microbiology · 2017Article
- Virtual microfluidics for digital quantification and single-cell sequencing.Nature methods · 2016Article
- Microfluidic Sample Preparation for Single Cell Analysis.Analytical chemistry · 2016Review
- Biophysical basis for convergent evolution of two veil-forming microbes.Royal Society open science · 2015Article
- Metabolic diversity and ecological niches of Achromatium populations revealed with single-cell genomic sequencing.Frontiers in microbiology · 2015Article
- Impact of single-cell genomics and metagenomics on the emerging view of extremophile "microbial dark matter".Extremophiles : life under extreme conditions · 2014Review
- Pan-genome analyses identify lineage- and niche-specific markers of evolution and adaptation in Epsilonproteobacteria.Frontiers in microbiology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We determined a significant fraction of the genome sequence of a representative of Thiovulum, the uncultivated genus of colorless sulfur Epsilonproteobacteria, by analyzing the genome sequences of four individual cells collected from phototrophic mats from Elkhorn Slough, California. These cells were isolated utilizing a microfluidic laser-tweezing system, and their genomes were amplified by multiple-displacement amplification prior to sequencing. Thiovulum is a gradient bacterium found at oxic-anoxic marine interfaces and noted for its distinctive morphology and rapid swimming motility. The genomic sequences of the four individual cells were assembled into a composite genome consisting of 221 contigs covering 2.083 Mb including 2,162 genes. This single-cell genome represents a genomic view of the physiological capabilities of isolated Thiovulum cells. Thiovulum is the second-fastest bacterium ever observed, swimming at 615 μm/s, and this genome shows that this rapid swimming motility is a result of a standard flagellar machinery that has been extensively characterized in other bacteria. This suggests that standard flagella are capable of propelling bacterial cells at speeds much faster than typically thought. Analysis of the genome suggests that naturally occurring Thiovulum populations are more diverse than previously recognized and that studies performed in the past probably address a wide range of unrecognized genotypic and phenotypic diversities of Thiovulum. The genome presented in this article provides a basis for future isolation-independent studies of Thiovulum, where single-cell and metagenomic tools can be used to differentiate between different Thiovulum genotypes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.