ArticleApplied and environmental microbiology2012
Characterization of a broad-specificity β-glucanase acting on β-(1,3)-, β-(1,4)-, and β-(1,6)-glucans that defines a new glycoside hydrolase family.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- Characterization of two GH10 enzymes with ability to hydrolyze pretreated Sorghum bicolor bagasse.Applied microbiology and biotechnology · 2025Article
- Crystal Structure of CcGH131B, a Protein Belonging to Glycoside Hydrolase Family 131 from the BasidiomyceteJournal of applied glycoscience · 2025Article
- Differential Carbon Catabolite Repression and Hemicellulolytic Ability among Pathotypes ofJournal of fungi (Basel, Switzerland) · 2024Article
- Secretome Analysis ofJournal of fungi (Basel, Switzerland) · 2024Article
- Combinatorial treatment with β-glucanase enzyme and chlorhexidine induces cysticidal effects in Acanthamoeba cyst.Parasitology research · 2022Article
- Transcriptome Analysis of Fusarium-Tomato Interaction Based on an Updated Genome Annotation ofJournal of fungi (Basel, Switzerland) · 2022Article
- Droplet digital microfluidic system for screening filamentous fungi based on enzymatic activity.Microsystems & nanoengineering · 2022Article
- Modulation of jejunal mucosa-associated microbiota in relation to intestinal health and nutrient digestibility in pigs by supplementation of β-glucanase to corn-soybean meal-based diets with xylanase.Journal of animal science · 2021Article
- Transcriptome analysis of the brown rot fungus Gloeophyllum trabeum during lignocellulose degradation.PloS one · 2020Article
- Evaluation of the Enzymatic Arsenal Secreted byFrontiers in bioengineering and biotechnology · 2020Article
- Genome description of Phlebia radiata 79 with comparative genomics analysis on lignocellulose decomposition machinery of phlebioid fungi.BMC genomics · 2019Article
- Following the terrestrial tracks of Caulobacter - redefining the ecology of a reputed aquatic oligotroph.The ISME journal · 2018Article
- Wheat ATI CM3, CM16 and 0.28 Allergens Produced inPlants (Basel, Switzerland) · 2018Article
- Coupling Secretomics with Enzyme Activities To Compare the Temporal Processes of Wood Metabolism among White and Brown Rot Fungi.Applied and environmental microbiology · 2018Article
- The β-glucosidase secreted byBiotechnology for biofuels · 2018Article
- Functional Analysis of a Novel β-(1,3)-Glucanase from Corallococcus sp. Strain EGB Containing a Fascin-Like Module.Applied and environmental microbiology · 2017Article
- Inactivation of Cellobiose Dehydrogenases Modifies the Cellulose Degradation Mechanism of Podospora anserina.Applied and environmental microbiology · 2017Article
- A Novel Glycoside Hydrolase Family 5 β-1,3-1,6-Endoglucanase from Saccharophagus degradans 2-40T and Its Transglycosylase Activity.Applied and environmental microbiology · 2016Article
- The Quaternary Structure of a Glycoside Hydrolase Dictates Specificity toward β-Glucans.The Journal of biological chemistry · 2016Article
- The N-Terminal GH10 Domain of a Multimodular Protein from Caldicellulosiruptor bescii Is a Versatile Xylanase/β-Glucanase That Can Degrade Crystalline Cellulose.Applied and environmental microbiology · 2015Article
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we report the cloning of the Pa_3_10940 gene from the coprophilic fungus Podospora anserina, which encodes a C-terminal family 1 carbohydrate binding module (CBM1) linked to a domain of unknown function. The function of the gene was investigated by expression of the full-length protein and a truncated derivative without the CBM1 domain in the yeast Pichia pastoris. Using a library of polysaccharides of different origins, we demonstrated that the full-length enzyme displays activity toward a broad range of β-glucan polysaccharides, including laminarin, curdlan, pachyman, lichenan, pustulan, and cellulosic derivatives. Analysis of the products released from polysaccharides revealed that this β-glucanase is an exo-acting enzyme on β-(1,3)- and β-(1,6)-linked glucan substrates and an endo-acting enzyme on β-(1,4)-linked glucan substrates. Hydrolysis of short β-(1,3), β-(1,4), and β-(1,3)/β-(1,4) gluco-oligosaccharides confirmed this striking feature and revealed that the enzyme performs in an exo-type mode on the nonreducing end of gluco-oligosaccharides. Excision of the CBM1 domain resulted in an inactive enzyme on all substrates tested. To our knowledge, this is the first report of an enzyme that displays bifunctional exo-β-(1,3)/(1,6) and endo-β-(1,4) activities toward beta-glucans and therefore cannot readily be assigned to existing Enzyme Commission groups. The amino acid sequence has high sequence identity to hypothetical proteins within the fungal taxa and thus defines a new family of glycoside hydrolases, the GH131 family.
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