ArticleNature nanotechnology2012
Determination of the elastic moduli of thin samples and adherent cells using conical atomic force microscope tips.
Article in Nature nanotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers, 1 of them a synthesis that pooled it.
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Who cites it
95 citing papers in PubMed, 1 synthesis or guideline pooled it, 305 citations in OpenAlex.
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- Nanoscale Investigation of Elasticity Changes and Augmented Rigidity of Block Copolymer Micelles Induced by Reversible Core-Cross-Linking.ACS applied materials & interfaces · 2025Article
- Nanomechanical characterization of soft nanomaterial using atomic force microscopy.Materials today. Bio · 2025Review
- Nanomechanics of cell-derived matrices as a functional read-out in collagen VI-related congenital muscular dystrophies.Journal of the Royal Society, Interface · 2025Article
- CellMAP: an open-source software tool to batch-process cell topography and stiffness maps collected with an atomic force microscope.BMC bioinformatics · 2025Article
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- Analytical methods in studying cell force sensing: principles, current technologies and perspectives.Regenerative biomaterials · 2025Review
- Sensing Biomechanical Alterations in Red Blood Cells of Type 1 Diabetes Patients: Potential Markers for Microvascular Complications.Biosensors · 2024Article
- Phase-separating peptide coacervates with programmable material properties for universal intracellular delivery of macromolecules.Nature communications · 2024Article
- An open-source combined atomic force microscope and optical microscope for mechanobiology studies.Heliyon · 2024Article
- Accurate Modelling of AFM Force-Indentation Curves with Blunted Indenters at Small Indentation Depths.Micromachines · 2024Article
- Side-view optical microscopy-assisted atomic force microscopy for thickness-dependent nanobiomechanics.Nanoscale advances · 2024Article
- Article
35 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The atomic force microscope can detect the mechanical fingerprints of normal and diseased cells at the single-cell level under physiological conditions. However, atomic force microscopy studies of cell mechanics are limited by the 'bottom effect' artefact that arises from the stiff substrates used to culture cells. Because cells adhered to substrates are very thin, this artefact makes cells appear stiffer than they really are. Here, we show an analytical correction that accounts for this artefact when conical tips are used for atomic force microscope measurements of thin samples. Our bottom effect cone correction (BECC) corrects the Sneddon's model, which is widely used to measure Young's modulus, E. Comparing the performance of BECC and Sneddon's model on thin polyacrylamide gels, we find that although Sneddon's model overestimates E, BECC yields E values that are thickness-independent and similar to those obtained on thick regions of the gel. The application of BECC to measurements on live adherent fibroblasts demonstrates a significant improvement on the estimation of their local mechanical properties.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.