ArticleThe Plant cell2012
Two direct targets of cytokinin signaling regulate symbiotic nodulation in Medicago truncatula.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed, 143 citations in OpenAlex.
- Transcriptomic Comparison of Soybean Roots Inoculated with Different Rhizobium Strains During Early Symbiosis.Plants (Basel, Switzerland) · 2026Article
- Small but big player: the important role of microRNAs in legume crops.Molecular genetics and genomics : MGG · 2026Review
- An NSP2-MYB module orchestrates flavonoid biosynthesis and nodule symbiosis.Current biology : CB · 2026Article
- Impacts of gene duplication in the evolution of symbiotic root nodule symbiosis in legumes.Frontiers in plant science · 2026Article
- Integrating multi-omics approaches to shape legume root system architecture under drought stress: a comprehensive review.Frontiers in plant science · 2026Review
- Integrated Transcriptomic and Biochemical Analyses Reveal the Root Development-Promoting Mechanism ofPlants (Basel, Switzerland) · 2025Article
- Comparative transcriptomics reveals differential carbohydrate and lipid metabolism in roots and nodules of Rhizobia-Inoculated alfalfa (Medicago sativa L.).BMC plant biology · 2025Article
- The role of cytokinin receptors in Arabidopsis thaliana seed development and how they affect the metabolomic profile.Planta · 2025Article
- Article
- Cytokinin: From autoclaved DNA to two-component signaling.The Plant cell · 2024Review
- Epigenetic weapons of plants against fungal pathogens.BMC plant biology · 2024Review
- Comprehensive Analysis ofGenes · 2022Article
- Temporal change in chromatin accessibility predicts regulators of nodulation in Medicago truncatula.BMC biology · 2022Article
- Right time, right place: The dynamic role of hormones in rhizobial infection and nodulation of legumes.Plant communications · 2022Review
- Development and characterization of nitrogen and phosphorus use efficiency responsive genic and miRNA derived SSR markers in wheat.Heredity · 2022Article
- Innovation and appropriation in mycorrhizal and rhizobial Symbioses.The Plant cell · 2022Article
- Function and Regulation of microRNA171 in Plant Stem Cell Homeostasis and Developmental Programing.International journal of molecular sciences · 2022Review
- Spatiotemporal cytokinin response imaging and ISOPENTENYLTRANSFERASE 3 function in Medicago nodule development.Plant physiology · 2022Article
- At the Root of Nodule Organogenesis: Conserved Regulatory Pathways Recruited by Rhizobia.Plants (Basel, Switzerland) · 2021Review
- Evolutionary and functional analysis of two-component system in chickpea reveals CaRR13, a TypeB RR, as positive regulator of symbiosis.Plant biotechnology journal · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokinin regulates many aspects of plant development, and in legume crops, this phytohormone is necessary and sufficient for symbiotic nodule organogenesis, allowing them to fix atmospheric nitrogen. To identify direct links between cytokinins and nodule organogenesis, we determined a consensus sequence bound in vitro by a transcription factor (TF) acting in cytokinin signaling, the nodule-enhanced Medicago truncatula Mt RR1 response regulator (RR). Among genes rapidly regulated by cytokinins and containing this so-called RR binding site (RRBS) in their promoters, we found the nodulation-related Type-A RR Mt RR4 and the Nodulation Signaling Pathway 2 (NSP2) TF. Site-directed mutagenesis revealed that RRBS cis-elements in the RR4 and NSP2 promoters are essential for expression during nodule development and for cytokinin induction. Furthermore, a microRNA targeting NSP2 (miR171 h) is also rapidly induced by cytokinins and then shows an expression pattern anticorrelated with NSP2. Other primary targets regulated by cytokinins depending on the Cytokinin Response1 (CRE1) receptor were a cytokinin oxidase/dehydrogenase (CKX1) and a basic Helix-Loop-Helix TF (bHLH476). RNA interference constructs as well as insertion of a Tnt1 retrotransposon in the bHLH gene led to reduced nodulation. Hence, we identified two TFs, NSP2 and bHLH476, as direct cytokinin targets acting at the convergence of phytohormonal and symbiotic cues.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.