ArticleNature2012
Androgenetic haploid embryonic stem cells produce live transgenic mice.
Article in Nature, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
What it found
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The trial behind it
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Who cites it
98 citing papers in PubMed.
- Pluripotent stem cell-specific imprinted genes identified using human uniparental-induced pluripotent stem cells.Journal of advanced research · 2026Article
- One-step generation of semi-cloned zebrafish carrying a defined genetic modification.Cell research · 2026Article
- Generation of modified cows and sheep from spermatid-like haploid embryonic stem cells.Nature biotechnology · 2026Article
- Metabolic Imbalance Triggers Adaptive Remodeling to Accelerate Diploidization in Murine Haploid Embryonic Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Generation of Biparental Bovine Embryos via Haploid Cell Injection (Semi-cloning) for Embryo Production with Predetermined Parental Genomes.Current protocols · 2026Article
- Analysis of Human Uniparental Embryonic Stem Cells Reveals New Putative Imprinted Loci.Cell proliferation · 2026Article
- FLNB and TTC26 regulate ciliary Hedgehog signaling to maintain intervertebral disc matrix homeostasis in adolescent idiopathic scoliosis.Genome biology · 2026Article
- Fertilization with Protamine-2 deficient sperm triggers abnormal pronucleus development and zygotic cleavage†.Biology of reproduction · 2026Article
- Chromosome length is constrained by spindle scaling to ensure faithful mitosis in mammals.Molecular systems biology · 2026Article
- Mitochondrial metabolic imbalance drives diploidization in mouse haploid embryonic stem cells via NADPH overload.Nature communications · 2026Article
- In Vitro Gamete Production in Mammals: Decades of Pioneering Approaches in Germ Cell and Stem Cell Biology Supporting Innovative Discovery and Expansion of Human Reproductive Potential.Molecular reproduction and development · 2025Review
- A Novel Cultivation System for Germ Cell Proliferation and Sustaining Whole Testicular Niche.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Fertile androgenetic mice generated by targeted epigenetic editing of imprinting control regions.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Male gamete copies to characterize genome inheritance and generate progenies.Scientific reports · 2025Article
- In vitro gametogenesis: Towards competent oocytes: Limitations and future improvements for generating oocytes from pluripotent stem cells in culture.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Overexpression of Let-7a mitigates diploidization in mouse androgenetic haploid embryonic stem cells.iScience · 2024Article
- Development and application of haploid embryonic stem cells.Stem cell research & therapy · 2024Review
- Haploid embryos and embryonic stem cells to produce offspring with predetermined parental genomes in cattle.Animal reproduction · 2024Article
- Transcriptome profiles and chromatin states in mouse androgenetic haploid embryonic stem cells.Cell proliferation · 2023Article
- Generation of a human haploid neural stem cell line for genome-wide genetic screening.World journal of stem cells · 2023Article
38 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
20 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Haploids and double haploids are important resources for studying recessive traits and have large impacts on crop breeding, but natural haploids are rare in animals. Mammalian haploids are restricted to germline cells and are occasionally found in tumours with massive chromosome loss. Recent success in establishing haploid embryonic stem (ES) cells in medaka fish and mice raised the possibility of using engineered mammalian haploid cells in genetic studies. However, the availability and functional characterization of mammalian haploid ES cells are still limited. Here we show that mouse androgenetic haploid ES (ahES) cell lines can be established by transferring sperm into an enucleated oocyte. The ahES cells maintain haploidy and stable growth over 30 passages, express pluripotent markers, possess the ability to differentiate into all three germ layers in vitro and in vivo, and contribute to germlines of chimaeras when injected into blastocysts. Although epigenetically distinct from sperm cells, the ahES cells can produce viable and fertile progenies after intracytoplasmic injection into mature oocytes. The oocyte-injection procedure can also produce viable transgenic mice from genetically engineered ahES cells. Our findings show the developmental pluripotency of androgenentic haploids and provide a new tool to quickly produce genetic models for recessive traits. They may also shed new light on assisted reproduction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.