ArticleLab on a chip2012
A novel microfluidic platform for high-resolution imaging of a three-dimensional cell culture under a controlled hypoxic environment.
Article in Lab on a chip, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
70 citing papers in PubMed, 1 citations in OpenAlex.
- A tumor-on-a-chip model reveals and targets reciprocal macrophage-NK cell crosstalk to advance immunotherapy screening.Microsystems & nanoengineering · 2026Article
- An oxygen gradient chamber with simple geometry for generating oxygen gradients in hydrogel-based cell cultures within a well-plate-compatible format.Frontiers in bioengineering and biotechnology · 2026Article
- Dynamic and functional changes in vascular endothelial cell monolayers under combined hypoxic and inflammatory stimuli.Scientific reports · 2025Article
- Automated T-Cell Proliferation in Lab-on-Chip Devices Integrating Microfluidics and Deep Learning-Based Image Analysis for Long-Term Experiments.Biosensors · 2025Article
- State-of-the-Art Organ-on-Chip Models and Designs for Medical Applications: A Systematic Review.Biomimetics (Basel, Switzerland) · 2025Review
- Cancer-on-a-chip for precision cancer medicine.Lab on a chip · 2025Review
- pH-Dependent migratory behaviors of neutrophil-like cells in a microfluidic device with controllability of dissolved gas concentrations.Scientific reports · 2024Article
- Hypoxia suppresses glucose-induced increases in collective cell migration in vascular endothelial cell monolayers.Scientific reports · 2024Article
- Cleanroom-Free Microfluidic Device for Natural Induction of Hypoxia in 2D and 3D Tumor Models.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Improving tumor microenvironment assessment in chip systems through next-generation technology integration.Frontiers in bioengineering and biotechnology · 2024Review
- Functional PDMS Elastomers: Bulk Composites, Surface Engineering, and Precision Fabrication.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Engineered organoids for biomedical applications.Advanced drug delivery reviews · 2023Review
- Preclinical Models and Technologies in Glioblastoma Research: Evolution, Current State, and Future Avenues.International journal of molecular sciences · 2023Review
- Exploring New Dimensions of Tumor Heterogeneity: The Application of Single Cell Analysis to Organoid-Based 3D In Vitro Models.Advanced healthcare materials · 2023Review
- Three-dimensional oxygen concentration monitoring in hydrogels using low-cost phosphorescence lifetime imaging for tissue engineering.Biomedical optics express · 2023Article
- Microfluidic platform for the reproduction of hypoxic vascular microenvironments.Scientific reports · 2023Article
- Enabling oxygen-controlled microfluidic cultures for spatiotemporal microbial single-cell analysis.Frontiers in microbiology · 2023Article
- Microfluidics in vascular biology research: a critical review for engineers, biologists, and clinicians.Lab on a chip · 2022Review
- Recapitulating Tumor Hypoxia in a Cleanroom-Free, Liquid-Pinning-Based Microfluidic Tumor Model.ACS biomaterials science & engineering · 2022Article
- Local extensional flows promote long-range fiber alignment in 3D collagen hydrogels.Biofabrication · 2022Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Low oxygen tensions experienced in various pathological and physiological conditions are a major stimulus for angiogenesis. Hypoxic conditions play a critical role in regulating cellular behaviour including migration, proliferation and differentiation. This study introduces the use of a microfluidic device that allows for the control of oxygen tension for the study of different three-dimensional (3D) cell cultures for various applications. The device has a central 3D gel region acting as an external cellular matrix, flanked by media channels. On each side, there is a peripheral gas channel through which suitable gas mixtures are supplied to establish a uniform oxygen tension or gradient within the device. The effects of various parameters, such as gas and media flow rates, device thickness, and diffusion coefficients of oxygen were examined using numerical simulations to determine the characteristics of the microfluidic device. A polycarbonate (PC) film with a low oxygen diffusion coefficient was embedded in the device in proximity above the channels to prevent oxygen diffusion from the incubator environment into the polydimethylsiloxane (PDMS) device. The oxygen tension in the device was then validated experimentally using a ruthenium-coated (Ru-coated) oxygen-sensing glass cover slip which confirmed the establishment of low uniform oxygen tensions (<3%) or an oxygen gradient across the gel region. To demonstrate the utility of the microfluidic device for cellular experiments under hypoxic conditions, migratory studies of MDA-MB-231 human breast cancer cells were performed. The microfluidic device allowed for imaging cellular migration with high-resolution, exhibiting an enhanced migration in hypoxia in comparison to normoxia. This microfluidic device presents itself as a promising platform for the investigation of cellular behaviour in a 3D gel scaffold under varying hypoxic conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.