ArticleFree radical biology & medicine2012
In vitro susceptibility of thioredoxins and glutathione to redox modification and aging-related changes in skeletal muscle.
Article in Free radical biology & medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- MitoQ supplementation does not impact redox responses to acute exercise in skeletal muscle of older individuals.Redox biology · 2025Trial
- Redox-regulated signalling of adaptations to contractile activity in skeletal muscle: Implications for age-related muscle weakness.Experimental physiology · 2025Review
- Mitochondria as Nutritional Targets to Maintain Muscle Health and Physical Function During Ageing.Sports medicine (Auckland, N.Z.) · 2024Review
- Endogenous and Exogenous Antioxidants in Skeletal Muscle Fatigue Development during Exercise.Antioxidants (Basel, Switzerland) · 2023Review
- Redox Control of Signalling Responses to Contractile Activity and Ageing in Skeletal Muscle.Cells · 2022Review
- Article
- Nicorandil decreases oxidative stress in slow- and fast-twitch muscle fibers of diabetic rats by improving the glutathione system functioning.Journal of diabetes investigation · 2021Article
- On the mechanisms underlying attenuated redox responses to exercise in older individuals: A hypothesis.Free radical biology & medicine · 2020Article
- Hydrogen peroxide as a signal for skeletal muscle adaptations to exercise: What do concentrations tell us about potential mechanisms?Redox biology · 2020Review
- HyPer2 imaging reveals temporal and heterogeneous hydrogen peroxide changes in denervated and aged skeletal muscle fibers in vivo.Scientific reports · 2019Article
- Long-Term Exercise Protects against Cellular Stresses in Aged Mice.Oxidative medicine and cellular longevity · 2018Article
- The p75BMC research notes · 2017Article
- Redox homeostasis and age-related deficits in neuromuscular integrity and function.Journal of cachexia, sarcopenia and muscle · 2017Review
- Redox Control of Skeletal Muscle Regeneration.Antioxidants & redox signaling · 2017Review
- MiR-23-TrxR1 as a novel molecular axis in skeletal muscle differentiation.Scientific reports · 2017Article
- Ageing-induced changes in the redox status of peripheral motor nerves imply an effect on redox signalling rather than oxidative damage.Free radical biology & medicine · 2016Article
- Age affects the contraction-induced mitochondrial redox response in skeletal muscle.Frontiers in physiology · 2015Article
- Interactions between reactive oxygen species generated by contractile activity and aging in skeletal muscle?Antioxidants & redox signaling · 2013Article
- Aging increases the oxidation of dichlorohydrofluorescein in single isolated skeletal muscle fibers at rest, but not during contractions.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Thioredoxins (Trx's) regulate redox signaling and are localized to various cellular compartments. Specific redox-regulated pathways for adaptation of skeletal muscle to contractions are attenuated during aging, but little is known about the roles of Trx's in regulating these pathways. This study investigated the susceptibility of Trx1 and Trx2 in skeletal muscle to oxidation and reduction in vitro and the effects of aging and contractions on Trx1, Trx2, and thioredoxin reductase (TrxR) 1 and 2 contents and nuclear and cytosolic Trx1 and mitochondrial Trx2 redox potentials in vivo. The proportions of cytosolic and nuclear Trx1 and mitochondrial Trx2 in the oxidized or reduced forms were analyzed using redox Western blotting. In myotubes, the mean redox potentials were nuclear Trx1, -251 mV; cytosolic Trx1, -242mV; mitochondrial Trx2, -346mV, data supporting the occurrence of differing redox potentials between cell compartments. Exogenous treatment of myoblasts and myotubes with hydrogen peroxide or dithiothreitol modified glutathione redox status and nuclear and cytosolic Trx1, but mitochondrial Trx2 was unchanged. Tibialis anterior muscles from young and old mice were exposed to isometric muscle contractions in vivo. Aging increased muscle contents of Trx1, Trx2, and TrxR2, but neither aging nor endogenous ROS generated during contractions modified Trx redox potentials, although oxidation of glutathione and other thiols occurred. We conclude that glutathione redox couples in skeletal muscle are more susceptible to oxidation than Trx and that Trx proteins are upregulated during aging, but do not appear to modulate redox-regulated adaptations to contractions that fail during aging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.