ArticleJournal of proteomics2012
A quantitative proteomic workflow for characterization of frozen clinical biopsies: laser capture microdissection coupled with label-free mass spectrometry.
Article in Journal of proteomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Proteomic characterization of circulating extracellular vesicles identifies novel serum myeloma associated markers.Journal of proteomics · 2016Trial
- edgeR v4: powerful differential analysis of sequencing data with expanded functionality and improved support for small counts and larger datasets.Nucleic acids research · 2025Article
- Rapid Multivariate Analysis Approach to Explore Differential Spatial Protein Profiles in Tissue.Journal of proteome research · 2023Article
- Global-feature of autoimmune glomerulonephritis using proteomic analysis of laser capture microdissected glomeruli.Frontiers in immunology · 2023Article
- A reference tissue atlas for the human kidney.Science advances · 2022Article
- Cellular and molecular interrogation of kidney biopsy specimens.Current opinion in nephrology and hypertension · 2022Review
- Differentiating Staphylococcus infection-associated glomerulonephritis and primary IgA nephropathy: a mass spectrometry-based exploratory study.Scientific reports · 2020Article
- FACS-Based Proteomics Enables Profiling of Proteins in Rare Cell Populations.International journal of molecular sciences · 2020Article
- Stimulation of retrotrapezoid nucleus Phox2b-expressing neurons rescues breathing dysfunction in an experimental Parkinson's disease rat model.Brain pathology (Zurich, Switzerland) · 2020Article
- Article
- How Omics Data Can Be Used in Nephrology.American journal of kidney diseases : the official journal of the National Kidney Foundation · 2018Review
- Proteomic profiling of human islets collected from frozen pancreata using laser capture microdissection.Journal of proteomics · 2017Article
- Tag-Count Analysis of Large-Scale Proteomic Data.Journal of proteome research · 2016Article
- A multi-model statistical approach for proteomic spectral count quantitation.Journal of proteomics · 2016Article
- Proteomic Signatures of Thymomas.PloS one · 2016Article
- Proteomics and autoimmune kidney disease.Clinical immunology (Orlando, Fla.) · 2015Review
- Laser capture microdissection: Big data from small samples.Histology and histopathology · 2015Review
- Mass spectrometry-based methods for identifying oxidized proteins in disease: advances and challenges.Biomolecules · 2015Review
- RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling.Journal of visualized experiments : JoVE · 2015Article
- Mass spectrometry identification of potential mediators of progestin-only contraceptive-induced abnormal uterine bleeding in human endometrial stromal cells.Contraception · 2015Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
This paper describes a simple, highly efficient and robust proteomic workflow for routine liquid-chromatography tandem mass spectrometry analysis of Laser Microdissection Pressure Catapulting (LMPC) isolates. Highly efficient protein recovery was achieved by optimization of a "one-pot" protein extraction and digestion allowing for reproducible proteomic analysis on as few as 500 LMPC isolated cells. The method was combined with label-free spectral count quantitation to characterize proteomic differences from 3000-10,000 LMPC isolated cells. Significance analysis of spectral count data was accomplished using the edgeR tag-count R package combined with hierarchical cluster analysis. To illustrate the capability of this robust workflow, two examples are presented: 1) analysis of keratinocytes from human punch biopsies of normal skin and a chronic diabetic wound and 2) comparison of glomeruli from needle biopsies of patients with kidney disease. Differentially expressed proteins were validated by use of immunohistochemistry. These examples illustrate that tissue proteomics carried out on limited clinical material can obtain informative proteomic signatures for disease pathogenesis and demonstrate the suitability of this approach for biomarker discovery.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.