ArticleBrain research bulletin2013
Ascorbic acid efficiently enhances neuronal synthesis of norepinephrine from dopamine.
Article in Brain research bulletin, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 75 citations in OpenAlex.
- A quantitative meta-analysis of vitamin C in the pathophysiology of Alzheimer's disease.Frontiers in aging neuroscience · 2022Pooled it
- Vitamin C supplementation promotes mental vitality in healthy young adults: results from a cross-sectional analysis and a randomized, double-blind, placebo-controlled trial.European journal of nutrition · 2022Trial
- Trial
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- Quercetin parallels ascorbic acid in neuroprotection against manganese-induced toxicity in Drosophila melanogaster.BMC complementary medicine and therapies · 2026Article
- Nutrients and bioactive compounds as modifiers of neurodegenerative trajectories: molecular mechanisms, translational barriers, and precision nutrition.Frontiers in nutrition · 2026Review
- Exploring the Epigenetic and Metabolic Pathways for Antioxidant and Anti-Inflammatory Potentials of Tart Cherry Juice Concentrate.Current pharmacology reports · 2025Review
- Nanolevel of detection of ascorbic acid using horse-radish peroxidase inhibition assay.Heliyon · 2024Article
- A dual-signal optical sensing platform of CDs-MnORSC advances · 2024Article
- The Interplay of Sports and Nutrition in Neurological Health and Recovery.Journal of clinical medicine · 2024Review
- Vitamin C protects retinal ganglion cells via SPP1 in glaucoma and after optic nerve damage.Life science alliance · 2023Article
- Vitamin C Modes of Action in Calcium-Involved Signaling in the Brain.Antioxidants (Basel, Switzerland) · 2023Review
- The effect of High-Dose Vitamin C Treatment for Acute Respiratory Failure Due to Coronavirus Disease Pneumonia on Mortality and Length of Intensive Care Stay: A Retrospective Cohort Study.Sisli Etfal Hastanesi tip bulteni · 2023Article
- A Review on Electrochemical Microsensors for Ascorbic Acid Detection: Clinical, Pharmaceutical, and Food Safety Applications.Micromachines · 2022Review
- Ultrasensitive dopamine detection with graphene aptasensor multitransistor arrays.Journal of nanobiotechnology · 2022Article
- Health behaviours and well-being among older adults with a Surinamese migration background in the Netherlands.BMC public health · 2022Article
- Neurodegenerative Diseases: Implications of Environmental and Climatic Influences on Neurotransmitters and Neuronal Hormones Activities.International journal of environmental research and public health · 2022Review
- The Efficacy of S-Adenosyl Methionine and Probiotic Supplementation on Depression: A Synergistic Approach.Nutrients · 2022Review
- Review
- A Case Report of a Modern-Day Scurvy.Kansas journal of medicine · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Ascorbic acid enhances synthesis of norepinephrine from dopamine in adrenal chromaffin cells by serving as a co-factor for chromaffin granule dopamine β-hydroxylase (DβH). However, there is controversy regarding in situ kinetics of the ascorbate effect in chromaffin cells, as well as whether they apply to neuronal cells. In this study we evaluated the stimulation of norepinephrine synthesis from dopamine in cultured SH-SY5Y neuroblastoma cells. These cells contained neither ascorbate nor norepinephrine in culture, but when provided with dopamine, they generated intracellular norepinephrine at rates that were stimulated several-fold by intracellular ascorbate. Ascorbate-induced increases in norepinephrine synthesis in dopamine-treated cells were linear over 60 min, despite saturation of intracellular ascorbate. Norepinephrine accumulation after 60 min of incubation with 100 μM dopamine was half-maximal at intracellular ascorbate concentrations of 0.2-0.5 mM, which fits well with the literature K(m) for ascorbate of DβH using dopamine as a substrate. Moreover, these ascorbate concentrations were generated by initial extracellular ascorbate concentrations of less than 25 μM due to concentrative accumulation by the ascorbate transporter. Treatment with 100 μM dopamine acutely increased cellular superoxide generation, which was prevented by ascorbate loading, but associated with a decrease in intracellular ascorbate when the latter was present at concentrations under 1 mM. These results show that ascorbate promptly enhances norepinephrine synthesis from dopamine by neuronal cells that it does so at physiologic intracellular concentrations in accord with the kinetics of DβH, and that it both protects cells from superoxide and by providing electrons to DβH.
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