ArticleMolecular cell2012
Membrane protein TM segments are retained at the translocon during integration until the nascent chain cues FRET-detected release into bulk lipid.
Article in Molecular cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Membrane Protein Insertion in Cells: Principles, Pathways, and Quality Control.Chemical reviews · 2026Review
- Tat-dependent protein translocation and its evolution.FEMS microbiology reviews · 2026Review
- ER-localized Shr3 is a selective co-translational folding chaperone necessary for amino acid permease biogenesis.The Journal of cell biology · 2023Article
- Folding and Insertion of Transmembrane Helices at the ER.International journal of molecular sciences · 2021Review
- CFTR trafficking mutations disrupt cotranslational protein folding by targeting biosynthetic intermediates.Nature communications · 2020Article
- Article
- Targeting and Insertion of Membrane Proteins.EcoSal Plus · 2017Review
- Protein export through the bacterial Sec pathway.Nature reviews. Microbiology · 2017Review
- Quality control of nonstop membrane proteins at the ER membrane and in the cytosol.Scientific reports · 2016Article
- The Ribosome-Sec61 Translocon Complex Forms a Cytosolically Restricted Environment for Early Polytopic Membrane Protein Folding.The Journal of biological chemistry · 2015Article
- Hydrophobic blocks facilitate lipid compatibility and translocon recognition of transmembrane protein sequences.Biochemistry · 2015Article
- SGTA regulates the cytosolic quality control of hydrophobic substrates.Journal of cell science · 2014Article
- Structural and functional profiling of the lateral gate of the Sec61 translocon.The Journal of biological chemistry · 2014Article
- Structures of the Sec61 complex engaged in nascent peptide translocation or membrane insertion.Nature · 2014Article
- Transmembrane segments form tertiary hairpins in the folding vestibule of the ribosome.Journal of molecular biology · 2014Article
- Article
- Reorientation of the first signal-anchor sequence during potassium channel biogenesis at the Sec61 complex.The Biochemical journal · 2013Article
- Functional asymmetry within the Sec61p translocon.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- YidC occupies the lateral gate of the SecYEG translocon and is sequentially displaced by a nascent membrane protein.The Journal of biological chemistry · 2013Article
- Reconciling the roles of kinetic and thermodynamic factors in membrane-protein insertion.Journal of the American Chemical Society · 2013Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Most membrane proteins are integrated cotranslationally into the ER membrane at the translocon, where nonpolar nascent protein transmembrane segments (TMSs) are widely believed to partition directly into the nonpolar membrane interior. However, a FRET approach that monitors the separation between a fluorescent-labeled TMS and fluorescent phospholipids diffusing in the bulk lipid reveals that TMSs do not immediately enter the lipid phase of the membrane. Instead, TMSs are retained at the translocon by protein-protein interactions until their release into bulk lipid is triggered by translation termination or, in some cases, by the arrival of another nascent chain TMS at a translocon. Nascent chain status and structural elements therefore dictate the timing of TMS release into the lipid phase by altering TMS and flanking sequence interactions with translocons, ribosomes, and associated proteins, thereby controlling when successive TMSs assemble in the bilayer and TMS-delineated loops fold.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.