ArticleStructure (London, England : 1993)2012
Movies of ice-embedded particles enhance resolution in electron cryo-microscopy.
Article in Structure (London, England : 1993), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 153 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
153 citing papers in PubMed.
- Article
- LowDoseWizard: rapid and standardized setup of low-dose cryo-TEM imaging in SerialEM.Acta crystallographica. Section D, Structural biology · 2026Article
- Laser phase plate improves structure determination of small proteins by cryo-EM.Science (New York, N.Y.) · 2026Article
- Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.eLife · 2026Article
- A single residue in the yellow fever virus envelope protein modulates virion architecture and antigenicity.Nature communications · 2025Article
- Advancing Cryo-EM and Cryo-ET through Innovation in Sample Carriers: A Perspective.Analytical chemistry · 2025Review
- TomoScore: A Neural Network Approach for Quality Assessment of Cellular cryo-ET.Research square · 2025Article
- Intraflagellar transport trains can switch rails and move along multiple microtubules in intact primary cilia.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- TomoScore: A Neural Network Approach for Quality Assessment of Cellular cryo-ET.bioRxiv : the preprint server for biology · 2024Article
- Cryo-EM phase-plate images reveal unexpected levels of apparent specimen damage.bioRxiv : the preprint server for biology · 2024Article
- Use of phase plate cryo-EM reveals conformation diversity of therapeutic IgG with 50 kDa Fab fragment resolved below 6 Å.Scientific reports · 2024Article
- Review
- Overcoming resolution attenuation during tilted cryo-EM data collection.Nature communications · 2024Article
- Structural Analysis of Protein Complexes by Cryo-Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Review
- A minority of final stacks yields superior amplitude in single-particle cryo-EM.Nature communications · 2023Article
- Application of Monolayer Graphene to Cryo-Electron Microscopy Grids for High-resolution Structure Determination.Journal of visualized experiments : JoVE · 2023Article
- Application of monolayer graphene to cryo-electron microscopy grids for high-resolution structure determination.bioRxiv : the preprint server for biology · 2023Article
- A large expert-curated cryo-EM image dataset for machine learning protein particle picking.Scientific data · 2023Article
- CryoPPP: A Large Expert-Labelled Cryo-EM Image Dataset for Machine Learning Protein Particle Picking.bioRxiv : the preprint server for biology · 2023Article
- Electron microscopy holdings of the Protein Data Bank: the impact of the resolution revolution, new validation tools, and implications for the future.Biophysical reviews · 2022Review
93 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors.
Funding
Abstract
Low-dose images obtained by electron cryo-microscopy (cryo-EM) are often affected by blurring caused by sample motion during electron beam exposure, degrading signal especially at high resolution. We show here that we can align frames of movies, recorded with a direct electron detector during beam exposure of rotavirus double-layered particles, thereby greatly reducing image blurring caused by beam-induced motion and sample stage instabilities. This procedure increases the efficiency of cryo-EM imaging and enhances the resolution obtained in three-dimensional reconstructions of the particle. Using movies in this way is generally applicable to all cryo-EM samples and should also improve the performance of midrange electron microscopes that may have limited mechanical stability and beam coherence.
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