ArticleGastrointestinal endoscopy2012
Near-infrared-labeled peptide multimer functions as phage mimic for high affinity, specific targeting of colonic adenomas in vivo (with videos).
Article in Gastrointestinal endoscopy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- A Novel Peptide Multimer for Enhanced Imaging and Multivalent Detection of Hepatocellular Carcinoma.Sensors and actuators. B, Chemical · 2025Article
- Membrane Bound Peroxiredoxin-1 Serves as a Biomarker forAntioxidants & redox signaling · 2022Article
- Multi-Modal Imaging Probe for Glypican-3 Overexpressed in Orthotopic Hepatocellular Carcinoma.Journal of medicinal chemistry · 2021Article
- Endoscopic molecular imaging in inflammatory bowel disease.Intestinal research · 2021Review
- Detection of colonic neoplasia in vivo using near-infrared-labeled peptide targeting cMet.Scientific reports · 2019Article
- Epidermal growth factor receptor-targeted molecular imaging of colorectal tumors: Detection and treatment evaluation of tumors in animal models.Cancer science · 2019Article
- Identification and validation of FGFR2 peptide for detection of early Barrett's neoplasia.Oncotarget · 2017Article
- Molecular Imaging of Colorectal Tumors by Targeting Colon Cancer Secreted Protein-2 (CCSP-2).Neoplasia (New York, N.Y.) · 2017Article
- Detection of Sessile Serrated Adenomas in the Proximal Colon Using Wide-Field Fluorescence Endoscopy.Gastroenterology · 2017Article
- Endoscopy-guided orthotopic implantation of colorectal cancer cells results in metastatic colorectal cancer in mice.Clinical & experimental metastasis · 2016Article
- Overexpressed Claudin-1 Can Be Visualized Endoscopically in Colonic Adenomas In Vivo.Cellular and molecular gastroenterology and hepatology · 2016Article
- Design and Synthesis of Near-Infrared Peptide for in Vivo Molecular Imaging of HER2.Bioconjugate chemistry · 2016Article
- Advanced gastrointestinal endoscopic imaging for inflammatory bowel diseases.World journal of gastroenterology · 2016Review
- Diagnostic imaging advances in murine models of colitis.World journal of gastroenterology · 2016Review
- From the surface to the single cell: Novel endoscopic approaches in inflammatory bowel disease.World journal of gastroenterology · 2015Review
- EGFR Overexpressed in Colonic Neoplasia Can be Detected on Wide-Field Endoscopic Imaging.Clinical and translational gastroenterology · 2015Article
- Molecular imaging in gastroenterology.Nature reviews. Gastroenterology & hepatology · 2013Review
- Optical molecular imaging for diagnosing intestinal diseases.Clinical endoscopy · 2013Review
- Endoscopic molecular imaging: status and future perspective.Clinical endoscopy · 2013Review
- Molecular imaging in endoscopy.United European gastroenterology journal · 2013Article
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5 authors.
Funding
Abstract
backgroundFluorescent-labeled peptides are being developed to improve the endoscopic detection of colonic dysplasia.
objectiveTo demonstrate a near-infrared peptide multimer that functions as a phage mimic for in vivo detection of colonic adenomas.
designA peptide multimer was synthesized by using trilysine as a dendritic wedge to mimic the presentation of peptides on phage, and all peptides, including the multimer, were fluorescent-labeled with Cy5.5.
settingSmall-animal imaging facility. ANIMAL SUBJECTS: Genetically engineered CPC;Apc mice that spontaneously develop colonic adenomas.
interventionNear-infrared-labeled AKPGYLS peptide multimer was administered topically into the distal colons of the mice, and endoscopic images of adenomas were captured. Fluorescence intensities were quantified by target-to-background (T/B) ratios, and adenoma dimensions were measured with calipers after imaging. Validation of specific peptide binding was performed on cryosectioned specimens and cells by using confocal microscopy and flow cytometry. MAIN OUTCOME MEASUREMENTS: Fluorescence T/B ratios from colonic adenomas and adjacent normal-appearing mucosa.
resultsAKP-multimer, monomer, trilysine core, and Cy5.5 resulted in mean (± SD) T/B ratios of 3.85 ± 0.25, 2.21 ± 0.13, 1.56 ± 0.12, and 1.19 ± 0.11, respectively, P < .01 on in vivo imaging. Peptide multimer showed higher contrast and greater specificity for dysplastic crypts as compared with other probes. Peptide multimer demonstrated significantly greater binding to HT29 cells on flow cytometry and fluorescence microscopy in comparison to monomer and trilysine core. A binding affinity of 6.4 nm/L and time constant of 0.1136 minutes(-1) (8.8 minutes) was measured for multimer. LIMITATIONS: Only distal colonic adenomas were imaged.
conclusionPeptide multimers combine strengths of multiple individual peptides to enhance binding interactions and demonstrate significantly higher specificity and affinity for tumor targets.
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