ArticleChemistry Central journal2012
Improvement of Catalytic Efficiency, Thermo-stability and Dye Decolorization Capability of Pleurotus ostreatus IBL-02 laccase by Hydrophobic Sol Gel Entrapment.
Article in Chemistry Central journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 73 citations in OpenAlex.
- Optimization, purification and characterization of laccase from a new endophytic Trichoderma harzianum AUMC14897 isolated from Opuntia ficus-indica and its applications in dye decolorization and wastewater treatment.Microbial cell factories · 2024Article
- Heterogeneous biocatalytic system for effective decolorization of textile dye effluent.3 Biotech · 2023Review
- Characterization of a Novel Laccase LAC-Yang1 from White-Rot FungusMolecules (Basel, Switzerland) · 2021Article
- Purification and immobilization of laccase fromJournal, genetic engineering & biotechnology · 2017Article
- Enzyme-based solutions for textile processing and dye contaminant biodegradation-a review.Environmental science and pollution research international · 2017Article
- Bio-based degradation of emerging endocrine-disrupting and dye-based pollutants using cross-linked enzyme aggregates.Environmental science and pollution research international · 2017Article
- Article
- Immobilized Lignin Peroxidase-Like Metalloporphyrins as Reusable Catalysts in Oxidative Bleaching of Industrial Dyes.Molecules (Basel, Switzerland) · 2016Review
- Dye decolorization and detoxification potential of Ca-alginate beads immobilized manganese peroxidase.BMC biotechnology · 2015Article
- Immobilized laccase mediated dye decolorization and transformation pathway of azo dye acid red 27.Journal of environmental health science & engineering · 2015Article
- Immobilization as a strategy for improving enzyme properties-application to oxidoreductases.Molecules (Basel, Switzerland) · 2014Review
- Enhanced decolorization of Solar brilliant red 80 textile dye by an indigenous white rot fungus Schizophyllum commune IBL-06.Saudi journal of biological sciences · 2013Article
- Decolorization applicability of sol-gel matrix immobilized manganese peroxidase produced from an indigenous white rot fungal strain Ganoderma lucidum.BMC biotechnology · 2013Article
- Degradation of textile dyes using immobilized lignin peroxidase-like metalloporphines under mild experimental conditions.Chemistry Central journal · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
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backgroundIn serious consideration of the worldwide environmental issues associated with the extensive use of the textile dyes and effluents generated thereof, the scientists across the world are in search for potential treatment technologies for their treatment. In such scenario the ligninolytic enzymes provide a potential alternative because they are cost effective, eco-friendly and can be applied to wide range of dye containing industrial effluents.
resultsLaccase produced from Pleurotus ostreatus IBL-02 during decolorization of the reactive textile dye Drimarene brilliant red K-4BL (DBR K-4BL) was purified and immobilized by hydrophobic gel entrapment. The crude laccase was 4.2-fold purified with specific activity of 573.52 U/mg after passing through the DEAE-Sepharose ion exchange and Sephadex-G-100 chromatography columns. P. ostreatus IBL-02 laccase was found to be a homogenous monomeric protein as evident by single band corresponding to 67 kDa on native and sodium dodesylsulfate polyacrylamide gel electrophoresis (PAGE). The laccase was immobilized by entrapment in Sol-gel matrix of trimethoxysilane (T) and proplytetramethoxysilane (P) prepared using different T:P molar ratios. The free and immobilized laccases were compared to investigate the effect of immobilization on catalytic efficiency and thermo-stability features. Laccase immobilized in the Sol-gel of 1:5 T:P ratio was optimally active and thermo-stable fraction at pH 5, 60°C with half-life of 3 h and 50 min. Laccases immobilized in 1:2 and 1:5 T:P ratio gels had significantly higher Km (83 and100mM) and Vmax (1000 and 1111 mM/mg) values as compared to free laccase. After 5 h reaction time varying decolorization percentages with a maximum of 100% were achieved for different dyes and effluents.
conclusionsIn summary, P. ostreatus IBL-02 laccase was immobilized by entrapping in a Sol-gel matrix with an objective to enhance its catalytic and stability properties. Sol-gel entrapped laccase presented potential efficiency as a biocatalyst when applied for decolorization of different dyes and effluents. The main benefits of the Sol-gel matrix immobilization processes are the eco-friendly approach, chemical free and energy saving reaction conditions.
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