ArticleBMC molecular biology2012
Adenylate kinase 2 (AK2) promotes cell proliferation in insect development.
Article in BMC molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 23 citations in OpenAlex.
- Regulation of Adenine Nucleotide Metabolism by Adenylate Kinase Isozymes: Physiological Roles and Diseases.International journal of molecular sciences · 2023Review
- Association between host genetics of sheep and the rumen microbial composition.Tropical animal health and production · 2022Article
- The Effect of the Protein Synthesis Entropy Reduction on the Cell Size Regulation and Division Size of Unicellular Organisms.Entropy (Basel, Switzerland) · 2022Article
- Long non-coding RNA BANCR promotes interferon-β-induced cardiomyocyte apoptosis by targeting signal transducer and activator of transcription 1International journal of clinical and experimental pathology · 2020Article
- A model for reticular dysgenesis shows impaired sensory organ development and hair cell regeneration linked to cellular stress.Disease models & mechanisms · 2019Article
- AK2 deficiency compromises the mitochondrial energy metabolism required for differentiation of human neutrophil and lymphoid lineages.Cell death & disease · 2015Article
- Reticular dysgenesis-associated AK2 protects hematopoietic stem and progenitor cell development from oxidative stress.The Journal of experimental medicine · 2015Article
- Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing.Frontiers in physiology · 2015Review
- Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAdenylate kinase 2 (AK2) is a phosphotransferase that catalyzes the reversible reaction 2ADP(GDP) ↔ ATP(GTP) + AMP and influences cellular energy homeostasis. However, the role of AK2 in regulating cell proliferation remains unclear because AK2 has been reported to be involved in either cell proliferation or cell apoptosis in different cell types of various organisms.
resultsThis study reports AK2 promotion of cell proliferation using the lepidopteran insect Helicoverpa armigera and its epidermal cell line HaEpi as models. Western blot analysis indicates that AK2 constitutively expresses in various tissues during larval development. Immunocytochemistry analysis indicates that AK2 localizes in the mitochondria. The recombinant expressed AK2 in E. coli promotes cell growth and viability of HaEpi cell line by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. AK2 knockdown in larvae by RNA interference causes larval growth defects, including body weight decrease and development delay. AK2 knockdown in larvae also decreases the number of circulating haemocytes. The mechanism for such effects might be the suppression of gene transcription involved in insect development caused by AK2 knockdown.
conclusionThese results show that AK2 regulates cell growth, viability, and proliferation in insect growth and development.
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