ArticleAnalytical chemistry2012
Global multi-method analysis of affinities and cooperativity in complex systems of macromolecular interactions.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Multiple data set Bayesian analysis synergistically boosts ITC parameter precision.Biophysical journal · 2026Article
- Integrative biophysical characterization of molecular interactions: A case study with the sfGFP-nanobody complex.Analytical biochemistry · 2025Article
- Assembly of SARS-CoV-2 nucleocapsid protein with nucleic acid.Nucleic acids research · 2024Article
- Assembly reactions of SARS-CoV-2 nucleocapsid protein with nucleic acid.bioRxiv : the preprint server for biology · 2023Article
- A conserved oligomerization domain in the disordered linker of coronavirus nucleocapsid proteins.Science advances · 2023Article
- E3 ligase autoinhibition by C-degron mimicry maintains C-degron substrate fidelity.Molecular cell · 2023Article
- Biophysical Characterization of Membrane Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Biophysical and functional study of CRL5Scientific reports · 2022Article
- Energetic and structural features of SARS-CoV-2 N-protein co-assemblies with nucleic acids.iScience · 2021Article
- Global multi-method analysis of interaction parameters for reversibly self-associating macromolecules at high concentrations.Scientific reports · 2021Article
- Energetic and structural features of SARS-CoV-2 N-protein co-assemblies with nucleic acids.bioRxiv : the preprint server for biology · 2021Article
- Unravelling the intricate cooperativity of subunit gating in P2X2 ion channels.Scientific reports · 2020Article
- Measuring the affinity of protein-protein interactions on a single-molecule level by mass photometry.Analytical biochemistry · 2020Article
- Nucleic acid-induced dimerization of HIV-1 Gag protein.The Journal of biological chemistry · 2019Article
- Positive Cooperativity in Substrate Binding by Human Thymidylate Synthase.Biophysical journal · 2019Article
- Cooperative assembly of a four-molecule signaling complex formed upon T cell antigen receptor activation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Congratulations to Dr. Fumio Arisaka on his 70th birthday.Biophysical reviews · 2018Article
- Polarization spectroscopy methods in the determination of interactions of small molecules with nucleic acids - tutorial.Beilstein journal of organic chemistry · 2018Review
- Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions.Nature protocols · 2017Article
- Measuring Protein Interactions by Optical Biosensors.Current protocols in protein science · 2017Article
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2 authors.
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Abstract
Cooperativity, multisite, and multicomponent interactions are hallmarks of biological systems of interacting macromolecules. Their thermodynamic characterization is often very challenging due to the notoriously low information content of binding isotherms. We introduce a strategy for the global multimethod analysis of data from multiple techniques (GMMA) that exploits enhanced information content emerging from the mutual constraints of the simultaneous modeling of orthogonal observables from calorimetric, spectroscopic, hydrodynamic, biosensing, or other thermodynamic binding experiments. We describe new approaches to address statistical problems that arise in the analysis of dissimilar data sets. The GMMA approach can significantly increase the complexity of interacting systems that can be accurately thermodynamically characterized.
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