ArticleProceedings of the National Academy of Sciences of the United States of America2012
Tetrameric assembly of KvLm K+ channels with defined numbers of voltage sensors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Lipid liquid-crystalline nanoparticles as a suitable platform for accommodating sensitive membrane proteins: monitoring the activity of HMG-CoA reductase.Journal of nanobiotechnology · 2025Article
- The PLB measurement for the connector in Phi29 bacteriophage reveals the function of its channel loop.Biophysical journal · 2021Article
- New Structures and Gating of Voltage-Dependent Potassium (Kv) Channels and Their Relatives: A Multi-Domain and Dynamic Question.International journal of molecular sciences · 2019Review
- Functional characterization of Kv11.1 (hERG) potassium channels split in the voltage-sensing domain.Pflugers Archiv : European journal of physiology · 2018Article
- A new mechanism of voltage-dependent gating exposed by KThe Journal of general physiology · 2017Article
- In vitro synthesis of a Major Facilitator Transporter for specific active transport across Droplet Interface Bilayers.Scientific reports · 2016Article
- Grafting voltage and pharmacological sensitivity in potassium channels.Cell research · 2016Article
- The Sensorless Pore Module of Voltage-gated K+ Channel Family 7 Embodies the Target Site for the Anticonvulsant Retigabine.The Journal of biological chemistry · 2016Article
- Lipid bilayer modules as determinants of K+ channel gating.The Journal of biological chemistry · 2014Article
- Stabilization of the conductive conformation of a voltage-gated K+ (Kv) channel: the lid mechanism.The Journal of biological chemistry · 2013Article
- Cell-free protein expression systems in microdroplets: Stabilization of interdroplet bilayers.Biomicrofluidics · 2013Article
- Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes.Scientific reports · 2013Article
- Identification and functional characterization of the Piezo1 channel pore domain.The Journal of biological chemistryArticle
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Authors and funding
4 authors.
Funding
Abstract
Voltage-gated K(+) (Kv) channels are tetrameric assemblies in which each modular subunit consists of a voltage sensor and a pore domain. KvLm, the voltage-gated K(+) channel from Listeria monocytogenes, differs from other Kv channels in that its voltage sensor contains only three out of the eight charged residues previously implicated in voltage gating. Here, we ask how many sensors are required to produce a functional Kv channel by investigating heterotetramers comprising combinations of full-length KvLm (FL) and its sensorless pore module. KvLm heterotetramers were produced by cell-free expression, purified by electrophoresis, and shown to yield functional channels after reconstitution in droplet interface bilayers. We studied the properties of KvLm channels with zero, one, two, three, and four voltage sensors. Three sensors suffice to promote channel opening with FL(4)-like voltage dependence at depolarizing potentials, but all four sensors are required to keep the channel closed during membrane hyperpolarization.
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