ArticleProceedings of the National Academy of Sciences of the United States of America2012
Single-cell analysis of early B-lymphocyte development suggests independent regulation of lineage specification and commitment in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed.
- B-lineage commitment is dependent on a reversible epigenetic switch.Genes & development · 2026Article
- miRNA-mRNA network analysis identifies PAX5 as a potential regulator of adaptive immune response in COPD.bioRxiv : the preprint server for biology · 2025Article
- Early B-Cell Factor 1: An Archetype for a Lineage-Restricted Transcription Factor Linking Development to Disease.Advances in experimental medicine and biology · 2024Review
- From Genotype to Phenotype: How Enhancers Control Gene Expression and Cell Identity in Hematopoiesis.HemaSphere · 2023Review
- Transcription factor networks link B-lymphocyte development and malignant transformation in leukemia.Genes & development · 2023Review
- A regulatory network of microRNAs confers lineage commitment during early developmental trajectories of B and T lymphocytes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Multiomics uncovers developing immunological lineages in human.European journal of immunology · 2021Review
- Single-Cell Analysis Reveals Regulatory Gene Expression Dynamics Leading to Lineage Commitment in Early T Cell Development.Cell systems · 2019Article
- Programming for T-lymphocyte fates: modularity and mechanisms.Genes & development · 2019Review
- Molecular Regulation of Differentiation in Early B-Lymphocyte Development.International journal of molecular sciences · 2018Review
- Identification of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome-associated DNA methylation patterns.PloS one · 2018Article
- Interaction of CCR4-NOT with EBF1 regulates gene-specific transcription and mRNA stability in B lymphopoiesis.Genes & development · 2016Article
- Hematopoiesis and T-cell specification as a model developmental system.Immunological reviews · 2016Review
- Mechanisms of fate decision and lineage commitment during haematopoiesis.Immunology and cell biology · 2016Review
- Transcription factor networks in B-cell differentiation link development to acute lymphoid leukemia.Blood · 2015Review
- Combined heterozygous loss of Ebf1 and Pax5 allows for T-lineage conversion of B cell progenitors.The Journal of experimental medicine · 2015Article
- The transcription repressors Bach2 and Bach1 promote B cell development by repressing the myeloid program.Nature immunology · 2014Article
- The regulatory network of B-cell differentiation: a focused view of early B-cell factor 1 function.Immunological reviews · 2014Review
- Transcriptional control of early T and B cell developmental choices.Annual review of immunology · 2014Review
- The Interplay between Chromatin and Transcription Factor Networks during B Cell Development: Who Pulls the Trigger First?Frontiers in immunology · 2014Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To better understand the process of B-lymphocyte lineage restriction, we have investigated molecular and functional properties in early B-lineage cells from Pax-5-deficient animals crossed to a B-lineage-restricted reporter mouse, allowing us to identify B-lineage-specified progenitors independently of conventional surface markers. Pax-5 deficiency resulted in a dramatic increase in the frequency of specified progenitor B-cells marked by expression of a λ5 (Igll1) promoter-controlled reporter gene. Gene expression analysis of ex vivo isolated progenitor cells revealed that Pax-5 deficiency has a minor impact on B-cell specification. However, single-cell in vitro differentiation analysis of ex vivo isolated cells revealed that specified B-lineage progenitors still displayed a high degree of plasticity for development into NK or T lineage cells. In contrast, we were unable to detect any major changes in myeloid lineage potential in specified Pax-5-deficient cells. By comparison of gene expression patterns in ex vivo isolated Pax-5- and Ebf-1-deficient progenitors, it was possible to identify a set of B-cell-restricted genes dependent on Ebf-1 but not Pax-5, supporting the idea that B-cell specification and commitment is controlled by distinct regulatory networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.