ArticleNucleic acids research2012
Evolutionarily divergent spliceosomal snRNAs and a conserved non-coding RNA processing motif in Giardia lamblia.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- Taxonomy of introns and the evolution of minor introns.Nucleic acids research · 2024Article
- Spliceosomal introns in the diplomonad parasiteMicrobial genomics · 2023Article
- Spliceosome assembly and regulation: insights from analysis of highly reduced spliceosomes.RNA (New York, N.Y.) · 2023Review
- Emerging biology of noncoding RNAs in malaria parasites.PLoS pathogens · 2022Review
- The role of nuclear organization in trans-splicing based expression of heat shock protein 90 in Giardia lamblia.PLoS neglected tropical diseases · 2021Article
- The compact genome ofMicrobial genomics · 2020Article
- Recent advances in functional research in Giardia intestinalis.Advances in parasitology · 2020Review
- Patterns of conservation of spliceosomal intron structures and spliceosome divergence in representatives of the diplomonad and parabasalid lineages.BMC evolutionary biology · 2019Article
- The cellular landscape of mid-size noncoding RNA.Wiley interdisciplinary reviews. RNA · 2019Review
- Nucleomorph Small RNAs in Cryptophyte and Chlorarachniophyte Algae.Genome biology and evolution · 2019Article
- Robust and stable transcriptional repression in Giardia using CRISPRi.Molecular biology of the cell · 2019Article
- Spliceosomal introns in Trichomonas vaginalis revisited.Parasites & vectors · 2018Article
- Domestication of self-splicing introns during eukaryogenesis: the rise of the complex spliceosomal machinery.Biology direct · 2017Review
- Interactions between Giardia duodenalis Sm proteins and their association with spliceosomal snRNAs.Parasitology research · 2017Article
- Transcriptomic analysis of diplomonad parasites reveals a trans-spliced intron in a helicase gene inPeerJ · 2017Article
- Splicing diversity revealed by reduced spliceosomes in C. merolae and other organisms.RNA biology · 2015Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Non-coding RNAs (ncRNAs) have diverse essential biological functions in all organisms, and in eukaryotes, two such classes of ncRNAs are the small nucleolar (sno) and small nuclear (sn) RNAs. In this study, we have identified and characterized a collection of sno and snRNAs in Giardia lamblia, by exploiting our discovery of a conserved 12 nt RNA processing sequence motif found in the 3' end regions of a large number of G. lamblia ncRNA genes. RNA end mapping and other experiments indicate the motif serves to mediate ncRNA 3' end formation from mono- and di-cistronic RNA precursor transcripts. Remarkably, we find the motif is also utilized in the processing pathway of all four previously identified trans-spliced G. lamblia introns, revealing a common RNA processing pathway for ncRNAs and trans-spliced introns in this organism. Motif sequence conservation then allowed for the bioinformatic and experimental identification of additional G. lamblia ncRNAs, including new U1 and U6 spliceosomal snRNA candidates. The U6 snRNA candidate was then used as a tool to identity novel U2 and U4 snRNAs, based on predicted phylogenetically conserved snRNA-snRNA base-pairing interactions, from a set of previously identified G. lamblia ncRNAs without assigned function. The Giardia snRNAs retain the core features of spliceosomal snRNAs but are sufficiently evolutionarily divergent to explain the difficulties in their identification. Most intriguingly, all of these snRNAs show structural features diagnostic of U2-dependent/major and U12-dependent/minor spliceosomal snRNAs.
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