ArticleLab on a chip2012
Microfluidic extraction, stretching and analysis of human chromosomal DNA from single cells.
Article in Lab on a chip, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 58 citations in OpenAlex.
- Single-Cell Nucleus Extraction with Cellular Indexing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- DNA forensics at forty: the way forward.International journal of legal medicine · 2025Review
- Bridging the Gap between Vertebrate Cytogenetics and Genomics with Single-Chromosome Sequencing (ChromSeq).Genes · 2021Review
- 3D Printing-Enabled DNA Extraction for Long-Read Genomics.ACS omega · 2020Article
- Digital Microfluidics for Single Bacteria Capture and Selective Retrieval Using Optical Tweezers.Micromachines · 2020Article
- Transverse migration and microfluidic concentration of DNA using Newtonian buffers.Biomicrofluidics · 2019Article
- Microfluidic long DNA sample preparation from cells.Lab on a chip · 2019Article
- Single-molecule DNA-mapping and whole-genome sequencing of individual cells.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Transverse dielectrophoretic-based DNA nanoscale confinement.Scientific reports · 2018Article
- Hydrogel droplet single-cell processing: DNA purification, handling, release, and on-chip linearization.Biomicrofluidics · 2018Article
- Single cell on-chip whole genome amplification via micropillar arrays for reduced amplification bias.PloS one · 2018Article
- Microscopic origin of wall slip during flow of an entangled DNA solution in microfluidics: Flow induced chain stretching versus chain desorption.Biomicrofluidics · 2017Article
- Electrophoretic stretching and imaging of single native chromatin fibers in nanoslits.Biomicrofluidics · 2017Article
- Alternating current electrohydrodynamics in microsystems: Pushing biomolecules and cells around on surfaces.Biomicrofluidics · 2015Review
- Single molecule and single cell epigenomics.Methods (San Diego, Calif.) · 2015Review
- Cellular mechanosensing: getting to the nucleus of it all.Progress in biophysics and molecular biology · 2014Review
- Micro total analysis systems: fundamental advances and biological applications.Analytical chemistry · 2014Review
- Epigenomics: one molecule at a time.Cell cycle (Georgetown, Tex.) · 2013Article
- Micro- and nanoscale devices for the investigation of epigenetics and chromatin dynamics.Nature nanotechnology · 2013Review
- Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
We describe a microfluidic device for the extraction, purification and stretching of human chromosomal DNA from single cells. A two-dimensional array of micropillars in a microfluidic polydimethylsiloxane channel was designed to capture a single human cell. Megabase-long DNA strands released from the cell upon lysis are trapped in the micropillar array and stretched under optimal hydrodynamic flow conditions. Intact chromosomal DNA is entangled in the array, while other cellular components are washed from the channel. To demonstrate the entrapment principle, a single chromosome was hybridized to whole chromosome paints, and imaged by fluorescence microscopy. DNA extracted from a single cell and small cell populations (less than 100) was released from the device by restriction endonuclease digestion under continuous flow and collected for off-chip analysis. Quantification of the extracted material reveals that the microdevice efficiently extracts essentially all chromosomal DNA. The device described represents a novel platform to perform a variety of analyses on chromosomal DNA at the single cell level.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.