ArticleDiagnostic microbiology and infectious disease2012
Use of an improved quantitative polymerase chain reaction assay to determine differences in human rhinovirus viral loads in different populations.
Article in Diagnostic microbiology and infectious disease, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.
- Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections.Clinical microbiology reviews · 2019Guideline
- Comparison of asymptomatic and symptomatic rhinovirus infections in university students: incidence, species diversity, and viral load.Diagnostic microbiology and infectious disease · 2015Trial
- Vitamin D3 and gargling for the prevention of upper respiratory tract infections: a randomized controlled trial.BMC infectious diseases · 2014Trial
- Likelihood of hospitalization for a chronic respiratory condition following pediatric infection with enterovirus and rhinovirus strains.Infectious medicine · 2022Article
- Epidemiological analysis and follow-up of human rhinovirus infection in children with asthma exacerbation.Journal of medical virology · 2018Article
- Clinical and epidemiological profiles including meteorological factors of low respiratory tract infection due to human rhinovirus in hospitalized children.Italian journal of pediatrics · 2017Article
- Reliable quantification of rhinovirus species C using real-time PCR.Journal of virological methods · 2016Article
- Association between secondary thrombocytosis and viral respiratory tract infections in children.Scientific reports · 2016Article
- The impact of viral dynamics on the clinical severity of infants with respiratory syncytial virus bronchiolitis.Journal of medical virology · 2015Article
- Single detection of human bocavirus 1 with a high viral load in severe respiratory tract infections in previously healthy children.BMC infectious diseases · 2014Article
- Co-circulation of multiple genotypes of human rhinovirus during a large outbreak of respiratory illness in a veterans' long-term care home.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2013Article
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Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human rhinoviruses (HRV) frequently cause acute respiratory infections and chronic respiratory disease exacerbations. However, testing is not generally offered. We developed a modified HRV quantitative polymerase chain reaction (qPCR) assay to assess viral loads in the community and hospital patients. The assay had a lower limit of detection of 2 log(10) viral copies/mL and displayed linearity over 5 log(10) viral copies, with a lower limit of quantitation of 4 log(10) viral copies/mL. Mean viral loads (95% confidence interval) for hospitalized children, university students, and institutionalized elderly, were 7.08 log(10) viral copies/mL (6.7-7.5), 6.87 log(10) viral copies/mL (6.5-7.2), and 7.09 log(10) viral copies/mL (6.9-7.3), respectively (P = 0.67). Serial specimens of 14 university students showed a decrease of mean viral loads from 6.36 log(10) viral copies/mL on day 1 to 2.32 log(10) viral copies/mL 7 days past symptom onset (P < 0.001). Using an HRV qPCR, we showed that viral loads did not differ between the community and hospitalized populations and significantly decreased following symptoms onset in healthy individuals.
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Registered trials
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