ArticleJournal of neurochemistry2013
A nicotinic acetylcholine receptor transmembrane point mutation (G275E) associated with resistance to spinosad in Frankliniella occidentalis.
Article in Journal of neurochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 134 citations in OpenAlex.
- Evaluation of Field Variation in Flower Thrips,Insects · 2026Article
- Knockout of nAChR subunits in spider mites and their phytoseiid predators confers spinosyn cross-resistance and reveals a conserved mode of action in mites.Insect biochemistry and molecular biology · 2026Article
- Standing genetic variation underlies divergence of nicotinic acetylcholine receptor subunits among cryptic species of thebioRxiv : the preprint server for biology · 2025Article
- Methyl hexenoate and linalool emitted by hot peppers repel western flower thrips (Frankliniella occidentalis) and attract their predator (Orius laevigatus).Scientific reports · 2025Article
- The cys-loop ligand-gated ion channel gene superfamily of the Colorado potato beetle, Leptinotarsa decemlineata.BMC genomics · 2025Article
- Field-evolved resistance to neonicotinoids in the mosquito, Anopheles gambiae, is associated with mutations of nicotinic acetylcholine receptor subunits combined with cytochrome P450-mediated detoxification.Pesticide biochemistry and physiology · 2024Article
- Field-evolved resistance to neonicotinoids in the mosquito,bioRxiv : the preprint server for biology · 2024Article
- The modes of action of ion-channel-targeting neurotoxic insecticides: lessons from structural biology.Nature structural & molecular biology · 2023Review
- Phenotypic Profiling of Macrocyclic Lactones on ParasiticAntimicrobial agents and chemotherapy · 2023Article
- Article
- Article
- Genomic Signatures of Recent Adaptation in a Wild Bumblebee.Molecular biology and evolution · 2022Article
- Nicotinic acetylcholine receptor modulator insecticides act on diverse receptor subtypes with distinct subunit compositions.PLoS genetics · 2022Article
- Mutation (G275E) of nAChR subunit Foα6 associated with spinetoram resistance in Australian western flower thrips, Frankliniella occidentalis (Pergande).Molecular biology reports · 2021Article
- A De Novo Transcriptomics Approach Reveals Genes Involved in Thrips Tabaci Resistance to Spinosad.Insects · 2021Article
- Insecticide Rotation and Adaptive Fitness Cost Underlying Insecticide Resistance Management for Spodoptera frugiperda (Lepidoptera: Noctuidae).Neotropical entomology · 2020Article
- Evolutionary trade-offs of insecticide resistance - The fitness costs associated with target-site mutations in the nAChR of Drosophila melanogaster.Molecular ecology · 2020Article
- Multiple mutations in the nicotinic acetylcholine receptor Ccα6 gene associated with resistance to spinosad in medfly.Scientific reports · 2019Article
- Nicotinic acetylcholine receptor α6 subunit mutation (G275V) found in a spinosad-resistant strain of the flower thrips,Journal of pesticide science · 2018Article
- Spinosad resistance affects biological parameters of Musca domestica Linnaeus.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
High levels of resistance to spinosad, a macrocyclic lactone insecticide, have been reported previously in western flower thrips, Frankliniella occidentalis, an economically important insect pest of vegetables, fruit and ornamental crops. We have cloned the nicotinic acetylcholine receptor (nAChR) α6 subunit from F. occidentalis (Foα6) and compared the nucleotide sequence of Foα6 from susceptible and spinosad-resistant insect populations (MLFOM and R1S respectively). A single nucleotide change has been identified in Foα6, resulting in the replacement of a glycine (G) residue in susceptible insects with a glutamic acid (E) in resistant insects. The resistance-associated mutation (G275E) is predicted to lie at the top of the third α-helical transmembrane domain of Foα6. Although there is no direct evidence identifying the location of the spinosad binding site, the analogous amino acid in the C. elegans glutamate-gated chloride channel lies in close proximity (4.4 Å) to the known binding site of ivermectin, another macrocyclic lactone pesticide. The functional consequences of the resistance-associated mutation have been examined in the human nAChR α7 subunit. Introduction of an analogous (A272E) mutation in α7 abolishes the modulatory effects of spinosad whilst having no significant effect upon activation by acetylcholine, consistent with spinosad having an allosteric mechanism of action.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.