ArticleStem cells and development2013
Growth requirements and chromosomal instability of induced pluripotent stem cells generated from adult canine fibroblasts.
Article in Stem cells and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 62 citations in OpenAlex.
- Advancing stem cell technologies for conservation of wildlife biodiversity.Development (Cambridge, England) · 2024Review
- Opening the "Black Box" Underlying Barriers to the Use of Canine Induced Pluripotent Stem Cells: A Narrative Review.Stem cells and development · 2023Review
- Pluripotent Stem Cells of Order Carnivora: Technical Perspective.International journal of molecular sciences · 2023Review
- Genetic Basis of Dilated Cardiomyopathy in Dogs and Its Potential as a Bidirectional Model.Animals : an open access journal from MDPI · 2022Review
- iPSC Therapy for Myocardial Infarction in Large Animal Models: Land of Hope and Dreams.Biomedicines · 2021Review
- L-Cells · 2021Article
- Perspectives of pluripotent stem cells in livestock.World journal of stem cells · 2021Review
- Targeted expression profiling reveals distinct stages of early canine fibroblast reprogramming are regulated by 2-oxoglutarate hydroxylases.Stem cell research & therapy · 2020Article
- The use of induced pluripotent stem cells in domestic animals: a narrative review.BMC veterinary research · 2020Review
- Optimized Approaches for the Induction of Putative Canine Induced Pluripotent Stem Cells from Old Fibroblasts Using Synthetic RNAs.Animals : an open access journal from MDPI · 2020Article
- Oocytes, embryos and pluripotent stem cells from a biomedical perspective.Animal reproduction · 2019Article
- Induced pluripotent stem cells throughout the animal kingdom: Availability and applications.World journal of stem cells · 2019Review
- Safety and immune regulatory properties of canine induced pluripotent stem cell-derived mesenchymal stem cells.Stem cell research · 2017Article
- A novel canine kidney cell line model for the evaluation of neoplastic development: karyotype evolution associated with spontaneous immortalization and tumorigenicity.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Article
- Induced pluripotent stem cells: Mechanisms, achievements and perspectives in farm animals.World journal of stem cells · 2015Review
- Canine Pluripotent Stem Cells: Are They Ready for Clinical Applications?Frontiers in veterinary science · 2015Review
- Mesenchymal and induced pluripotent stem cells: general insights and clinical perspectives.Stem cells and cloning : advances and applications · 2015Review
- Comparison of American mink embryonic stem and induced pluripotent stem cell transcriptomes.BMC genomics · 2015Article
- Preclinical studies for induced pluripotent stem cell-based therapeutics.The Journal of biological chemistry · 2014Review
- From "ES-like" cells to induced pluripotent stem cells: a historical perspective in domestic animals.Theriogenology · 2014Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mice and humans, it has been shown that embryonic and adult fibroblasts can be reprogrammed into pluripotency by introducing 4 transcription factors, Oct3/4, Klf4, Sox2, and c-Myc (OKSM). Here, we report the derivation of induced pluripotent stem cells (iPSCs) from adult canine fibroblasts by retroviral OKSM transduction. The isolated canine iPSCs (ciPSCs) were expanded in 3 different culture media [fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), or FGF2 plus LIF]. Cells cultured in both FGF2 and LIF expressed pluripotency markers [POU5F1 (OCT4), SOX2, NANOG, and LIN28] and embryonic stem cell (ESC)-specific genes (PODXL, DPPA5, FGF5, REX1, and LAMP1) and showed strong levels of alkaline phosphatase expression. In vitro differentiation by formation of embryoid bodies and by directed differentiation generated cell derivatives of all 3 germ layers as confirmed by mRNA and protein expression. In vivo, the ciPSCs created solid tumors, which failed to reach epithelial structure formation, but expressed markers for all 3 germ layers. Array comparative genomic hybridization and chromosomal fluorescence in situ hybridization analyses revealed that while retroviral transduction per se did not result in significant DNA copy number imbalance, there was evidence for the emergence of low-level aneuploidy during prolonged culture or tumor formation. In summary, we were able to derive ciPSCs from adult fibroblasts by using 4 transcription factors. The isolated iPSCs have similar characteristics to ESCs from other species, but the exact cellular mechanisms behind their unique co-dependency on both FGF2 and LIF are still unknown.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.