ArticleMolecular biology of the cell2012
The complex interactions of Chs5p, the ChAPs, and the cargo Chs3p.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Pck2 association with the plasma membrane and efficient response of the cell integrity pathway require regulation of PI4P homeostasis by exomer.Open biology · 2024Article
- Multiple quality control mechanisms monitor yeast chitin synthase folding in the endoplasmic reticulum.Molecular biology of the cell · 2023Article
- Identification of chitin synthase activator in Aspergillus niger and its application in citric acid fermentation.Applied microbiology and biotechnology · 2022Article
- Chitin Synthesis in Yeast: A Matter of Trafficking.International journal of molecular sciences · 2022Review
- Exomer Is Part of a Hub Where Polarized Secretion and Ionic Stress Connect.Frontiers in microbiology · 2021Article
- The Functional Specialization of Exomer as a Cargo Adaptor During the Evolution of Fungi.Genetics · 2018Article
- Involvement of the exomer complex in the polarized transport of Ena1 required forMolecular biology of the cell · 2017Article
- Article
- Maintaining protein homeostasis: early and late endosomal dual recycling for the maintenance of intracellular pools of the plasma membrane protein Chs3.Molecular biology of the cell · 2016Article
- Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Cargo adaptors: structures illuminate mechanisms regulating vesicle biogenesis.Trends in cell biology · 2015Review
- Review
- Structural basis for membrane binding and remodeling by the exomer secretory vesicle cargo adaptor.Developmental cell · 2014Article
- Cofilin recruits F-actin to SPCA1 and promotes Ca2+-mediated secretory cargo sorting.The Journal of cell biology · 2014Article
- Distinct N-terminal regions of the exomer secretory vesicle cargo Chs3 regulate its trafficking itinerary.Frontiers in cell and developmental biology · 2014Article
- The exomer cargo adaptor features a flexible hinge domain.Structure (London, England : 1993) · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The exomer complex is a putative vesicle coat required for the direct transport of a subset of cargoes from the trans-Golgi network (TGN) to the plasma membrane. Exomer comprises Chs5p and the ChAPs family of proteins (Chs6p, Bud7p, Bch1p, and Bch2p), which are believed to act as cargo receptors. In particular, Chs6p is required for the transport of the chitin synthase Chs3p to the bud neck. However, how the ChAPs associate with Chs5p and recognize cargo is not well understood. Using domain-switch chimeras of Chs6p and Bch2p, we show that four tetratricopeptide repeats (TPRs) are involved in interaction with Chs5p. Because these roles are conserved among the ChAPs, the TPRs are interchangeable among different ChAP proteins. In contrast, the N-terminal and the central parts of the ChAPs contribute to cargo specificity. Although the entire N-terminal domain of Chs6p is required for Chs3p export at all cell cycle stages, the central part seems to predominantly favor Chs3p export in small-budded cells. The cargo Chs3p probably also uses a complex motif for the interaction with Chs6, as the C-terminus of Chs3p interacts with Chs6p and is necessary, but not sufficient, for TGN export.
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