ArticleJournal of clinical microbiology2012
Culture-dependent and -independent investigations of microbial diversity on urinary catheters.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Insights into urinary catheter colonisation and polymicrobial biofilms of Candida- bacteria under flow condition.Scientific reports · 2025Article
- Asymptomatic bacteriuria and urinary tract infection in geriatric inpatients after indwelling urinary catheter removal: a descriptive two-centre study.Infection prevention in practice · 2024Article
- Microbial education plays a crucial role in harnessing the beneficial properties of microbiota for infectious disease protection in Crassostrea gigas.Scientific reports · 2024Article
- Review
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- Biofilm and Equine Limb Wounds.Animals : an open access journal from MDPI · 2021Review
- Indwelling Device-Associated Biofilms in Critically Ill Cancer Patients-Study Protocol.Pathogens (Basel, Switzerland) · 2021Article
- Urethral Catheter Biofilms Reveal Plasticity in Bacterial Composition and Metabolism and Withstand Host Immune Defenses in Hypoxic Environment.Frontiers in medicine · 2021Article
- Profiles of the bacterial community in short-term indwelling urinary catheters by duration of catheterization and subsequent urinary tract infection.American journal of infection control · 2020Article
- Optimizing the Catheter Care and Maintenance Strategy of Short-Term Catheterization among Hospitalized Patients in Microbiological Approach.BioMed research international · 2020Article
- Do Mixed-Species Biofilms Dominate in Chronic Infections?-Need forFrontiers in cellular and infection microbiology · 2020Review
- Methods Used for the Eradication of Staphylococcal Biofilms.Antibiotics (Basel, Switzerland) · 2019Review
- Encrustations on ureteral stents from patients without urinary tract infection reveal distinct urotypes and a low bacterial load.Microbiome · 2019Article
- Fluorescent Capillary Electrophoresis Is Superior to Culture in DetectingJournal of clinical microbiology · 2019Article
- Molecular Techniques Complement Culture-Based Assessment of Bacteria Composition in Mixed Biofilms of Urinary Tract Catheter-Related Samples.Frontiers in microbiology · 2019Article
- Quantitative assessment of individual populations within polymicrobial biofilms.Scientific reports · 2018Article
- Rapid diagnostic assay for detection of cellulose in urine as biomarker for biofilm-related urinary tract infections.NPJ biofilms and microbiomes · 2018Article
- Optical scatter patterns facilitate rapid differentiation of Enterobacteriaceae on CHROMagar™ Orientation medium.Microbial biotechnology · 2016Article
- Similar Neutrophil-Driven Inflammatory and Antibacterial Responses in Elderly Patients with Symptomatic and Asymptomatic Bacteriuria.Infection and immunity · 2015Observational
- Article
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Authors and funding
7 authors.
Funding
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Abstract
Catheter-associated urinary tract infection is caused by bacteria, which ascend the catheter along its external or internal surface to the bladder and subsequently develop into biofilms on the catheter and uroepithelium. Antibiotic-treated bacteria and bacteria residing in biofilm can be difficult to culture. In this study we used culture-based and 16S rRNA gene-based culture-independent methods (fingerprinting, cloning, and pyrosequencing) to determine the microbial diversity of biofilms on 24 urinary catheters. Most of the patients were catheterized for <30 days and had undergone recent antibiotic treatment. In addition, the corresponding urine samples for 16 patients were cultured. We found that gene analyses of the catheters were consistent with cultures of the corresponding urine samples for the presence of bacteria but sometimes discordant for the identity of the species. Cultures of catheter tips detected bacteria more frequently than urine cultures and gene analyses; coagulase-negative staphylococci were, in particular, cultured much more often from catheter tips, indicating potential contamination of the catheter tips during sampling. The external and internal surfaces of 19 catheters were separately analyzed by molecular methods, and discordant results were found in six catheters, suggesting that bacterial colonization intra- and extraluminally may be different. Molecular analyses showed that most of the species identified in this study were known uropathogens, and infected catheters were generally colonized by one to two species, probably due to antibiotic usage and short-term catheterization. In conclusion, our data showed that culture-independent molecular methods did not detect bacteria from urinary catheters more frequently than culture-based methods.
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