ArticleJournal of clinical microbiology2012
Identification of and screening for human Helicobacter cinaedi infections and carriers via nested PCR.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Article
- Genome and pathogenicity analysis of Helicobacter mastomyrinus isolated from mice.Archives of microbiology · 2025Article
- Determining Infected Aortic Aneurysm Treatment Using Focused Detection of Helicobacter cinaedi.Emerging infectious diseases · 2022Article
- Article
- Infections With Enterohepatic Non-Frontiers in cellular and infection microbiology · 2021Review
- Article
- Genome Sequencing Verifies Relapsed Infection ofOpen forum infectious diseases · 2019Article
- Bacteremia Possibly Caused by Helicobacter cinaedi and Associated with Painful Erythema in Rheumatoid Arthritis with Malignant Lymphoma.Internal medicine (Tokyo, Japan) · 2018Article
- Multi-step genomic dissection of a suspected intra-hospital Helicobacter cinaedi outbreak.Microbial genomics · 2018Article
- A Case Of RecurrentJournal of bone and joint infection · 2018Article
- ChronicJAAD case reports · 2017Article
- Unusual manifestation of Helicobacter cinaedi infection: a case report of intracranial subdural empyema and bacteremia.BMC infectious diseases · 2017Article
- Article
- Graft placement with an omental flap for ruptured infective common iliac aneurysm in a patient with a continuous flow left ventricular assist device: alternative surgical approach avoiding driveline injury and pathogen identification by 16S ribosomal DNA gene analysis.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2016Article
- Epithelial Coculture and l-Lactate Promote Growth of Helicobacter cinaedi under H2-Free Aerobic Conditions.Applied and environmental microbiology · 2016Article
- The pathogenic potential of Helicobacter cinaedi isolated from non-human sources: adherence, invasion and translocation ability in polarized intestinal epithelial Caco-2 cells in vitro.The Journal of veterinary medical science · 2016Article
- Helicobacter cinaedi induced typhlocolitis in Rag-2-deficient mice.Helicobacter · 2015Article
- Helicobacter cinaedi infection in patients with diabetes: a case report.SpringerPlus · 2015Article
- First case of infective endocarditis caused by Helicobacter cinaedi.BMC infectious diseases · 2014Article
- Helicobacter cinaedi infection of abdominal aortic aneurysm, Japan.Emerging infectious diseases · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Helicobacter cinaedi is the most frequently reported enterohepatic Helicobacter species isolated from humans. Earlier research suggested that certain patients with H. cinaedi infection may remain undiagnosed or incorrectly diagnosed because of difficulties in detecting the bacteria by conventional culture methods. Here, we report a nested PCR assay that rapidly detects the cytolethal distending toxin gene (cdt) of H. cinaedi with high specificity and sensitivity. Specificity of the assay was validated by using different species of Helicobacter and Campylobacter, as well as known H. cinaedi-positive and -negative samples. The sensitivity of detection for the cdt gene in the assay was 10(2) CFU/ml urine or 10(2) CFU/10(5) infected RAW 264.7 cells. In an H. cinaedi-infected mouse model, the cdt gene of H. cinaedi was effectively detected via the assay with urine (6/7), stool (2/3), and blood (2/6) samples. Importantly, it detected H. cinaedi in blood, urine, and stool samples from one patient with a suspected H. cinaedi infection and three patients with known infections. The assay was further used clinically to follow up two H. cinaedi-infected patients after antibiotic treatment. Stool samples from these two patients evaluated by nested PCR after antibiotic therapy showed clearance of bacterial DNA. Finally, analysis of stool specimens from healthy volunteers showed occasional positive reactions (4/30) to H. cinaedi DNA, which suggests intestinal colonization by H. cinaedi in healthy subjects. In conclusion, this nested PCR assay may be useful for the rapid diagnosis, antimicrobial treatment evaluation, and epidemiological study of H. cinaedi infection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.