ArticlePlant molecular biology2012
Cloning and comparative analysis of carotenoid β-hydroxylase genes provides new insights into carotenoid metabolism in tetraploid (Triticum turgidum ssp. durum) and hexaploid (Triticum aestivum) wheat grains.
Article in Plant molecular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Distinct growth patterns in seedling and tillering wheat plants suggests a developmentally restricted role of HYD2 in salt-stress response.Plant cell reports · 2024Article
- A multi-omic resource of wheat seed tissues for nutrient deposition and improvement for human health.Scientific data · 2023Article
- Temporal transcriptome and metabolite analyses provide insights into the biochemical and physiological processes underlying endodormancy release in pistachio (Frontiers in plant science · 2023Article
- Genetic dissection of the roles of β-hydroxylases in carotenoid metabolism, photosynthesis, and plant growth in tetraploid wheat (Triticum turgidum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Genetic dissection of lutein content in common wheat via association and linkage mapping.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Does Plant Breeding for Antioxidant-Rich Foods Have an Impact on Human Health?Antioxidants (Basel, Switzerland) · 2022Review
- Mutant combinations of lycopene ɛ-cyclase and β-carotene hydroxylase 2 homoeologs increased β-carotene accumulation in endosperm of tetraploid wheat (Triticum turgidum L.) grains.Plant biotechnology journal · 2022Article
- Enrichment of provitamin A content in durum wheat grain by suppressing β-carotene hydroxylase 1 genes with a TILLING approach.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Ornithine decarboxylase genes contribute to S-RNase-independent pollen rejection.Plant physiology · 2021Article
- Linkage disequilibrium patterns, population structure and diversity analysis in a worldwide durum wheat collection including Argentinian genotypes.BMC genomics · 2021Article
- Assessing the Role ofFrontiers in nutrition · 2021Article
- Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9.Nature communications · 2020Article
- Carotenoids in Cereal Food Crops: Composition and Retention throughout Grain Storage and Food Processing.Plants (Basel, Switzerland) · 2019Review
- Carotenoid Pigment Content in Durum Wheat (Frontiers in plant science · 2019Review
- A Genome-Wide Association Study Reveals a Rich Genetic Architecture of Flour Color-Related Traits in Bread Wheat.Frontiers in plant science · 2018Article
- The carotenoid biosynthetic and catabolic genes in wheat and their association with yellow pigments.BMC genomics · 2017Article
- Distinct expression and function of carotenoid metabolic genes and homoeologs in developing wheat grains.BMC plant biology · 2016Article
- Carotenoids in Staple Cereals: Metabolism, Regulation, and Genetic Manipulation.Frontiers in plant science · 2016Review
- The lycopene β-cyclase plays a significant role in provitamin A biosynthesis in wheat endosperm.BMC plant biology · 2015Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Carotenoid β-hydroxylases attach hydroxyl groups to the β-ionone rings (β-rings) of carotenoid substrates, resulting in modified structures and functions of carotenoid molecules. We cloned and characterized two genes (each with three homeologs), HYD1 and HYD2, which encode β-hydroxylases in wheat. The results from bioinformatic and nested degenerate PCR analyses collectively suggest that HYD1 and HYD2 may represent the entire complement of non-heme di-iron β-hydroxylases in wheat. The homeologs of wheat HYDs exhibited major β-ring and minor ε-ring hydroxylation activities in carotenoid-accumulating E. coli strains. Distinct expression patterns were observed for different HYD genes and homeologs in vegetative tissues and developing grains of tetraploid and hexaploid wheat, suggesting their functional divergence and differential regulatory control in tissue-, grain development-, and ploidy-specific manners. An intriguing observation was that the expression of HYD1, particularly HYD-B1, reached highest levels at the last stage of tetraploid and hexaploid grain development, suggesting that carotenoids (at least xanthophylls) were still actively synthesized in mature grains. This result challenges the common perception that carotenoids are simply being turned over during wheat grain development after their initial biosynthesis at the early grain development stages. Overall, this improved understanding of carotenoid biosynthetic gene expression and carotenoid metabolism in wheat grains will contribute to the improvement of the nutritional value of wheat grains for human consumption.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.