Evidence map›Paper›PMID 23014973›Full record

ArticleJournal of bone and mineral metabolism2013

Retinoic acid regulates commitment of undifferentiated mesenchymal stem cells into osteoblasts and adipocytes.

Kunihiro Hisada, Kenji Hata, Fumitaka Ichida, Takuma Matsubara, Hideo Orimo, Tamaki Nakano, Hirohumi Yatani, Riko Nishimura, Toshiyuki Yoneda

Abstract read
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In one paragraph

Article in Journal of bone and mineral metabolism, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Role of vitamins beyond vitamin DInternational journal of molecular medicine · 2024
    Review
  6. Article
  7. Review
  8. Review
  9. Article
  10. [All-trans retinoic acid and vascular endothelial growth factor induced the directional osteogenic differentiation of mouse embryonic fibroblasts].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2020
    Article
  11. Article
  12. Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Identification of compounds that modulate retinol signaling using a cell-based qHTS assay.Toxicology in vitro : an international journal published in association with BIBRA · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Kunihiro HisadaDepartment of Molecular and Cellular Biochemistry, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Kenji Hata
Fumitaka Ichida
Takuma Matsubara
Hideo Orimo
Tamaki Nakano
Hirohumi Yatani
Riko Nishimura
Toshiyuki Yoneda

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Evidence indicates that the balance between osteoblastogenesis and adipogenesis of mesenchymal stem cells (MSCs) is regulated by several hormones, growth factors, and their downstream signaling cascades. Previous studies suggest that retinoic acid (RA) plays a role in osteoblastogenesis and adipogenesis. However, it is unknown whether RA regulates commitment of MSCs into osteoblasts and adipocytes. In this study, we investigated the role of RA in differentiation of MSCs using the C3H10T1/2 cell line. RA stimulated activity and expression of alkaline phosphatase (ALP) and upregulated activity of the ALP gene promoter. The effects of RA were further enhanced by bone morphogenetic protein 2 (BMP2) and resultant Smad signaling. Furthermore, overexpression of Runx2 and Msx2, critical transcription factors for bone formation and BMP2-dependent osteoblastogenesis, enhanced RA-dependent ALP activity. In view of these findings, RA likely stimulates osteoblast differentiation through the BMP2-Smad-Runx2/Msx2 pathway. In contrast, RA markedly inhibited BMP2-induced adipocyte differentiation, suppressing expression of peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer-binding protein (C/EBP)α and C/EBPδ, and inhibiting adipogenic function of C/EBPβ, C/EBPδ, and PPARγ. In conclusion, our data suggest that RA regulates commitment of MSCs into osteoblasts and adipocytes by controlling transcriptional regulators.

Indexed as

AdipocytesAlkaline PhosphataseAnimalsAntineoplastic AgentsBone Morphogenetic Protein 2CCAAT-Enhancer-Binding Protein-deltaCCAAT-Enhancer-Binding ProteinsCell DifferentiationCore Binding Factor Alpha 1 SubunitGene Expression RegulationHEK293 CellsHomeodomain ProteinsHumansMesenchymal Stem CellsMiceOsteoblastsAlkaline PhosphataseAntineoplastic AgentsBMP2 protein, humanBmp2 protein, mouseBone Morphogenetic Protein 2CCAAT-Enhancer-Binding Protein-deltaCCAAT-Enhancer-Binding ProteinsCEBPA protein, humanCEBPA protein, mouseCEBPD protein, humanCebpd protein, mouseCore Binding Factor Alpha 1 SubunitHomeodomain ProteinsMSX2 proteinPPAR gammaRUNX2 protein, humanRunx2 protein, mouseSmad ProteinsTretinoin

Identifiers

PMID23014973

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.