ArticleTissue engineering. Part A2013
In vivo bioluminescence imaging of cell differentiation in biomaterials: a platform for scaffold development.
Article in Tissue engineering. Part A, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Non-Invasive Longitudinal Bioluminescence Imaging of Human Mesoangioblasts in Bioengineered Esophagi.Tissue engineering. Part C, Methods · 2019Article
- Bioluminescence-mediated longitudinal monitoring of adipose-derived stem cells in a large mammal ex vivo organ culture.Scientific reports · 2015Article
- The substrate matters in the Raman spectroscopy analysis of cells.Scientific reports · 2015Article
- Postinfarction Functional Recovery Driven by a Three-Dimensional Engineered Fibrin Patch Composed of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells.Stem cells translational medicine · 2015Article
- Finding and tracing human MSC in 3D microenvironments with the photoconvertible protein Dendra2.Scientific reports · 2015Article
- Imaging Approaches in Functional Assessment of Implantable Myogenic Biomaterials and Engineered Muscle Tissue.European journal of translational myology · 2015Review
- Pre-clinical characterization of tissue engineering constructs for bone and cartilage regeneration.Annals of biomedical engineering · 2015Review
- A perspective on the clinical translation of scaffolds for tissue engineering.Annals of biomedical engineering · 2015Article
- Imaging strategies for tissue engineering applications.Tissue engineering. Part B, Reviews · 2015Review
- Fast-proliferating adipose tissue mesenchymal-stromal-like cells for therapy.Stem cells and development · 2014Article
- In vivo bioluminescent tracking of mesenchymal stem cells within large hydrogel constructs.Tissue engineering. Part C, Methods · 2014Article
- Endothelial differentiation of adipose tissue-derived mesenchymal stromal cells in glioma tumors: implications for cell-based therapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Imaging challenges in biomaterials and tissue engineering.Biomaterials · 2013Article
- The angiogenic properties of mesenchymal stem/stromal cells and their therapeutic potential.British medical bulletin · 2013Review
- Application of bioluminescence resonance energy transfer-based cell tracking approach in bone tissue engineering.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo testing is a mandatory last step in scaffold development. Agile longitudinal noninvasive real-time monitoring of stem cell behavior in biomaterials implanted in live animals should facilitate the development of scaffolds for tissue engineering. We report on a noninvasive bioluminescence imaging (BLI) procedure for simultaneous monitoring of changes in the expression of multiple genes to evaluate scaffold performance in vivo. Adipose tissue-derived stromal mensenchymal cells were dually labeled with Renilla red fluorescent protein and firefly green fluorescent protein chimeric reporters regulated by cytomegalovirus and tissue-specific promoters, respectively. Labeled cells were induced to differentiate in vitro and in vivo, by seeding in demineralized bone matrices (DBMs) and monitored by BLI. Imaging results were validated by RT-polymerase chain reaction and histological procedures. The proposed approach improves molecular imaging and measurement of changes in gene expression of cells implanted in live animals. This procedure, applicable to the simultaneous analysis of multiple genes from cells seeded in DBMs, should facilitate engineering of scaffolds for tissue repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.