ArticleSensors (Basel, Switzerland)2012
A unifying review of bioassay-guided fractionation, effect-directed analysis and related techniques.
Article in Sensors (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed.
- A quantitative screening strategy based on activity-content ratio for prioritizing bioactive compounds in medicinal plants.Journal of pharmaceutical analysis · 2026Article
- Bioassay-guided fractionation and identification of wound healing active compounds from Khaya senegalensis leaves.PloS one · 2026Article
- Article
- Bioactive compound identification without fractionation: an Ocimum spp. case study.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Recent Advances in Understanding the Sex Pheromone-Mediated Communication of Diatoms.ChemPlusChem · 2025Review
- Unlocking potent anti-tuberculosis natural products through structure-activity relationship analysis.Natural products and bioprospecting · 2025Review
- Bridging Ethnobotanical Knowledge and Multi-Omics Approaches for Plant-Derived Natural Product Discovery.Metabolites · 2025Review
- Assessing the impact of environmental laws and technological advancements on carbon dioxide mitigation in China's mining industry.PloS one · 2025Article
- Primed for Discovery.Biochemistry · 2024Review
- Antifungal Potential of Secondary Metabolites Derived fromMolecules (Basel, Switzerland) · 2024Article
- Article
- Integrating High-Resolution Mass Spectral Data, Bioassays and Computational Models to Annotate Bioactives in Botanical Extracts: Case Study Analysis ofMolecules (Basel, Switzerland) · 2024Article
- Design and Synthesis of d-Ala-d-Ala Silica Gel for a Binding Mode-Based Physicochemical Screening Method.ACS omega · 2023Article
- Codeine dysregulates ribosome biogenesis inFuture drug discovery · 2023Article
- Anti-Inflammatory Effect of Ebractenoid F, a Major Active Compound ofPlants (Basel, Switzerland) · 2023Article
- Molecular networking identifies an AHR-modulating benzothiazole from white button mushrooms (Journal of functional foods · 2023Article
- Advances in PAH mixture toxicology enabled by zebrafish.Current opinion in toxicology · 2023Article
- Antimicrobial and Cytotoxic Activity of Endophytic Fungi fromJournal of microbiology and biotechnology · 2023Article
- Antioxidative-, Antimicrobial-, Enzyme Inhibition-, and Cytotoxicity-Based Fractionation and Isolation of Active Components fromACS omega · 2022Article
- A Metabolomic Study ofMetabolites · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The success of modern methods in analytical chemistry sometimes obscures the problem that the ever increasing amount of analytical data does not necessarily give more insight of practical relevance. As alternative approaches, toxicity- and bioactivity-based assays can deliver valuable information about biological effects of complex materials in humans, other species or even ecosystems. However, the observed effects often cannot be clearly assigned to specific chemical compounds. In these cases, the establishment of an unambiguous cause-effect relationship is not possible. Effect-directed analysis tries to interconnect instrumental analytical techniques with a biological/biochemical entity, which identifies or isolates substances of biological relevance. Successful application has been demonstrated in many fields, either as proof-of-principle studies or even for complex samples. This review discusses the different approaches, advantages and limitations and finally shows some practical examples. The broad emergence of effect-directed analytical concepts might lead to a true paradigm shift in analytical chemistry, away from ever growing lists of chemical compounds. The connection of biological effects with the identification and quantification of molecular entities leads to relevant answers to many real life questions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.