ArticleActa histochemica et cytochemica2012
Immunohistochemical Examination of Novel Rat Monoclonal Antibodies against Mouse and Human Podoplanin.
Article in Acta histochemica et cytochemica, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 73 citations in OpenAlex.
- PMab-322: a novel anti-hippopotamus podoplanin monoclonal antibody for multiple applications.Biochemistry and biophysics reports · 2025Article
- Endothelial eNOS deficiency causes podocyte injury through NFAT2 and heparanase in diabetic mice.Scientific reports · 2024Article
- Antipodoplanin antibody enhances the antitumor effects of CTLA-4 blockade against malignant mesothelioma by natural killer cells.Cancer science · 2024Article
- Review
- Novel circulating tumor cell-detection chip combining conventional podoplanin and EGFR antibodies for all histological malignant pleural mesothelioma.Oncology letters · 2021Article
- Site-specific epitope insertion into recombinant proteins using the MAP tag system.Journal of biochemistry · 2020Article
- Epitope Analysis of an Anti-Whale Podoplanin Monoclonal Antibody, PMab-237, Using Flow Cytometry.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2020Article
- Establishment of a monoclonal antibody PMab-233 for immunohistochemical analysis against Tasmanian devil podoplanin.Biochemistry and biophysics reports · 2019Article
- PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin.Biochemistry and biophysics reports · 2019Article
- Establishment of a monoclonal antibody PMab-225 against alpaca podoplanin for immunohistochemical analyses.Biochemistry and biophysics reports · 2019Article
- Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis.Biochemistry and biophysics reports · 2019Article
- PMab-235: A monoclonal antibody for immunohistochemical analysis against goat podoplanin.Heliyon · 2019Article
- Chloride intracellular channel protein 2 in cancer and non-cancer human tissues: relationship with tight junctions.Tissue barriers · 2019Article
- Epitope mapping of anti-mouse podoplanin monoclonal antibody PMab-1.Biochemistry and biophysics reports · 2018Article
- Expression and Dynamics of Podoplanin in Cultured Osteoblasts with Mechanostress and Mineralization Stimulus.Acta histochemica et cytochemica · 2018Article
- Development and characterization of anti-glycopeptide monoclonal antibodies against human podoplanin, using glycan-deficient cell lines generated by CRISPR/Cas9 and TALEN.Cancer medicine · 2017Article
- MAP Tag: A Novel Tagging System for Protein Purification and Detection.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2016Article
- Article
- Altered dynamics in the renal lymphatic circulation of type 1 and type 2 diabetic mice.Acta histochemica et cytochemica · 2013Article
- Expression of Toll-Like Receptor 4 in Glomerular Endothelial Cells under Diabetic Conditions.Acta histochemica et cytochemica · 2013Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aims to develop new monoclonal antibodies (mAbs) against mouse and human podoplanin. Rats were immunized with synthetic peptides, corresponding to amino acids 38-51 of mouse podoplanin or human podoplanin which is 100% homologous to the same site of monkey podoplanin; anti-mouse podoplanin mAb PMab-1 (IgG(2) (a)) and anti-human mAb NZ-1.2 (IgG(2) (a)) were established. In immunocytochemistry, the mouse melanoma B16-F10 and mouse podoplanin (mPDPN)-expressed CHO transfectant were stained by PMab-1; human lymphatic endothelial cells (LEC) and human podoplanin (hPDPN)-expressed squamous cell carcinoma HSC3 transfectant, were stained by NZ-1.2. Western-blot analysis detected an about 40-kDa protein in CHO-mPDPN and B16-F10 by PMab-1, and in HSC3-hPDPN and LEC by NZ-1.2. In frozen sections, PMab-1 reacted with mouse kidney, pulmonary alveoli, pulmonary pleura, and salivary gland myoepithelial cells while NZ-1.2 reacted to the human salivary gland myoepithelial cells. The immunostaining of paraffin-embedded sections also showed the reaction of PMab-1 or NZ-1.2 to the mouse or monkey kidney glomerulus, pulmonary alveoli, and lung lymphatic vessels. These results indicate that the two novel rat mAbs to the mouse and human/monkey podoplanin are useful for Western-blot and immunostaining of somatic tissues on paraffin-embedded sections as well as frozen sections.
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