ArticleProceedings of the National Academy of Sciences of the United States of America2012
bHLH-PAS heterodimer of methoprene-tolerant and Cycle mediates circadian expression of juvenile hormone-induced mosquito genes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 135 citations in OpenAlex.
- miR-318 regulates juvenile hormone biosynthesis to control ovarian development in Bactrocera dorsalis.Communications biology · 2026Article
- Characterization of the Crustacean Methyl Farnesoate Transcriptional Signaling Genes.International journal of molecular sciences · 2026Article
- Alternative Characterizations of Methyl Lucidone-Responsive Differentially Expressed Genes inInsects · 2025Article
- 20E-inducedProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- The seminal vesicle is a juvenile hormone-responsive tissue in adult maleOpen biology · 2024Article
- Article
- Neural mechanism of circadian clock-based photoperiodism in insects and snails.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2024Review
- Article
- Steroid receptor coactivator TAIMAN is a new modulator of insect circadian clock.PLoS genetics · 2023Article
- Overexpression ofInternational journal of molecular sciences · 2023Article
- Article
- Evolution of proteins involved in the final steps of juvenile hormone synthesis.Journal of insect physiology · 2023Article
- Juvenile hormone receptor Methoprene tolerant: Functions and applications.Vitamins and hormones · 2023Article
- Interaction patterns of methoprene-tolerant and germ cell-expressedFrontiers in molecular biosciences · 2023Article
- Endocrine Regulation of Lifespan in Insect Diapause.Frontiers in physiology · 2022Review
- Purification of an insect juvenile hormone receptor complex enables insights into its post-translational phosphorylation.The Journal of biological chemistry · 2021Article
- Krüppel-homolog 1 exerts anti-metamorphic and vitellogenic functions in insects via phosphorylation-mediated recruitment of specific cofactors.BMC biology · 2021Article
- Detection of juvenile hormone agonists by a new reporter gene assay using yeast expressing Drosophila methoprene-tolerant.FEBS open bio · 2021Article
- The vitellogenin receptor functionality of the migratory locust depends on its phosphorylation by juvenile hormone.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Juvenile hormone (JH) governs a great diversity of processes in insect development and reproduction. It plays a critical role in controlling the gonadotrophic cycles of female mosquitoes by preparing tissues for blood digestion and egg development. Here, we show that in female Aedes aegypti mosquitoes JH III control of gene expression is mediated by a heterodimer of two bHLH-PAS proteins-the JH receptor methoprene-tolerant (MET) and Cycle (CYC, AAEL002049). We identified Aedes CYC as a MET-interacting protein using yeast two-hybrid screening. Binding of CYC and MET required the presence of JH III. In newly eclosed female mosquitoes, the expression of two JH-responsive genes, Kr-h1 and Hairy, was dependent on both the ratio of light to dark periods and JH III. Their expression was compromised by in vivo RNA interference (RNAi) depletions of CYC, MET, and the steroid receptor coactivator SRC/FISC. Moreover, JH III was not effective in induction of Kr-h1 and Hairy gene expression in vitro in fat bodies of female mosquitoes with RNAi-depleted CYC, MET or SRC/FISC. A sequence containing an E-box-like motif from the Aedes Kr-h1 gene promoter specifically interacted with a protein complex, which included MET and CYC from the female mosquito fat body nuclear extract. These results indicate that a MET/CYC heterodimer mediates JH III activation of Kr-h1 and Hairy genes in the context of light-dependent circadian regulation in female mosquitoes during posteclosion development. This study provides an important insight into the understanding of the molecular basis of JH action.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.