ArticleLab on a chip2012
Studies of bacterial aerotaxis in a microfluidic device.
Article in Lab on a chip, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- Harnessing microfluidics for microbiology: from bacteria-host interactions to emerging cancer therapies.Communications biology · 2026Review
- An Overview of Microfluidic Phenotype Separation of Bacteria.Current pharmaceutical design · 2026Review
- Spatial self-organization of confined bacterial suspensions.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A standard 96-well based high throughput microfluidic perfusion biofilm reactor for in situ optical analysis.Biomedical microdevices · 2023Article
- Microfluidic devices for studying bacterial taxis, drug testing and biofilm formation.Microbial biotechnology · 2022Review
- Aggregation Temperature of Escherichia coli Depends on Steepness of the Thermal Gradient.Biophysical journal · 2020Article
- Microfluidic techniques for separation of bacterial cells via taxis.Microbial cell (Graz, Austria) · 2020Review
- Measuring and regulating oxygen levels in microphysiological systems: design, material, and sensor considerations.The Analyst · 2019Review
- Integrated phosphorescence-based photonic biosensor (iPOB) for monitoring oxygen levels in 3D cell culture systems.Biosensors & bioelectronics · 2019Article
- Spatial self-organization resolves conflicts between individuality and collective migration.Nature communications · 2018Article
- A Platform for High-Throughput Assessments of Environmental Multistressors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Osmotaxis inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Logarithmic sensing inNPJ systems biology and applications · 2017Article
- Aerotaxis in the closest relatives of animals.eLife · 2016Article
- The Aerotactic Response of Caulobacter crescentus.Biophysical journal · 2016Article
- The role of motility and chemotaxis in the bacterial colonization of protected surfaces.Scientific reports · 2016Article
- Oxygen Tension and Riboflavin Gradients Cooperatively Regulate the Migration ofFrontiers in microbiology · 2016Article
- Live from under the lens: exploring microbial motility with dynamic imaging and microfluidics.Nature reviews. Microbiology · 2015Review
- A novel microfluidic platform for studying mammalian cell chemotaxis in different oxygen environments under zero-flow conditions.Biomicrofluidics · 2015Article
- Single-Cell Physiology.Annual review of biophysics · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Aerotaxis, the directional motion of bacteria in gradients of oxygen, was discovered in the late 19th century and has since been reported in a variety of bacterial species. Nevertheless, quantitative studies of aerotaxis have been complicated by the lack of tools for generation of stable gradients of oxygen concentration, [O(2)]. Here we report a series of experiments on aerotaxis of Escherichia coli in a specially built experimental setup consisting of a computer-controlled gas mixer and a two-layer microfluidic device made of polydimethylsiloxane (PDMS). The setup enables generation of a variety of stable linear profiles of [O(2)] across a long gradient channel, with characteristic [O(2)] ranging from aerobic to microaerobic conditions. A suspension of E. coli cells is perfused through the gradient channel at a low speed, allowing cells enough time to explore the [O(2)] gradient, and the distribution of cells across the gradient channel is analyzed near the channel outlet at a throughput of >10(5) cells per hour. Aerotaxis experiments are performed in [O(2)] gradients with identical logarithmic slopes and varying mean concentrations, as well as in gradients with identical mean concentrations and varying slopes. Experiments in gradients with [O(2)] ranging from 0 to ~11.5% indicate that, in contrast to some previous reports, E. coli cells do not congregate at some intermediate level of [O(2)], but rather prefer the highest accessible [O(2)]. The presented technology can be applied to studies of aerotaxis of other aerobic and microaerobic bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.