ArticleJournal of orthopaedic trauma2012
Mesenchymal stem cells facilitate fracture repair in an alcohol-induced impaired healing model.
Article in Journal of orthopaedic trauma, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Fracture healing: from molecular and cellular mechanisms to therapeutic strategies.Signal transduction and targeted therapy · 2026Review
- Timing for Intravenous Injection of Mesenchymal Stem Cells to Enhance Fracture Healing.Journal of functional biomaterials · 2026Article
- Craniofacial Fracture and Secretome: A Systematic Review.Plastic and reconstructive surgery. Global open · 2026Article
- Comparison of the effects of hydrogel and normal saline as carriers of MSC on fracture healing in a rat long bone fracture model.Journal of orthopaedic surgery and research · 2025Article
- A single acute alcohol intoxication before fracture insult causes long-term elevated systemic RANKL and OPG levels in young adult mice.Scientific reports · 2025Article
- Exogenously delivered iPSCs disrupt the natural repair response of endogenous MPCs after bone injury.Scientific reports · 2023Article
- Optimal concentration of mesenchymal stem cells for fracture healing in a rat model with long bone fracture.World journal of stem cells · 2022Article
- Multiplexed Molecular Imaging Strategy Integrated with RNA Sequencing in the Assessment of the Therapeutic Effect of Wharton's Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles for Osteoporosis.International journal of nanomedicine · 2021Article
- Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus.Stem cell research & therapy · 2020Article
- Impact of Alcohol on Bone Health, Homeostasis and Fracture repair.Current pathobiology reports · 2020Article
- Ethanol Inhibits Mesenchymal Stem Cell Osteochondral Lineage Differentiation Due in Part to an Activation of Forkhead Box Protein O-Specific Signaling.Alcoholism, clinical and experimental research · 2020Article
- Mesenchymal stem cell treatment attenuates liver and lung inflammation after ethanol intoxication and burn injury.Alcohol (Fayetteville, N.Y.) · 2019Article
- Mechanisms Underlying Normal Fracture Healing and Risk Factors for Delayed Healing.Current osteoporosis reports · 2019Review
- Innovative Biomaterials for Bone Regrowth.International journal of molecular sciences · 2019Review
- Mesenchymal Stem Cell Migration during Bone Formation and Bone Diseases Therapy.International journal of molecular sciences · 2018Review
- Effects of Episodic Alcohol Exposure on BMP2 Signaling During Tibia Fracture Healing.Journal of orthopaedic trauma · 2018Article
- Alcohol exposure decreases osteopontin expression during fracture healing and osteopontin-mediated mesenchymal stem cell migration in vitro.Journal of orthopaedic surgery and research · 2018Article
- Clinical and Research Approaches to Treat Non-union Fracture.Current osteoporosis reports · 2018Review
- Adenovirus-mediated bone morphogenetic protein-2 promotes osteogenic differentiation in human mesenchymal stem cellsExperimental and therapeutic medicine · 2017Article
- Adiponectin regulates BMSC osteogenic differentiation and osteogenesis through the Wnt/β-catenin pathway.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
objectivesClinical studies have shown alcohol to be a risk factor for traumatic orthopaedic injuries and for nonunion. Data from animal studies suggest that alcohol exposure inhibits fracture healing. This report presents a novel rodent model of impaired fracture healing caused by repeated alcohol exposure. Using this model, we examined the regenerative effects of an intravenously administered population of isolated and expanded mesenchymal stem cells (MSCs) on fracture healing.
methodsBone marrow-derived MSC were isolated from transgenic green fluorescent protein C57BL/6 mice, and culture expanded using a lineage depletion protocol. Adult wild-type C57BL/6 mice were subjected to a 2-week binge alcohol exposure paradigm (3 days during which they received daily intraperitoneal injections of a 20% alcohol/saline solution followed by a 4-day rest period and another binge cycle for 3 consecutive days). At completion of the second binge cycle, mice were subjected to a mid-shaft tibia fracture while intoxicated. Twenty-four hours after the fracture, animals were administered an intravenous transplant of green fluorescent protein-labeled MSC. Two weeks after the fracture, animals were euthanized and injured tibiae were collected and subjected to biomechanical, histologic, and microcomputed tomography analysis.
resultsPre-injury binge alcohol exposure resulted in a significant impairment in biomechanical strength and decrease in callus volume. MSC transplants restored both fracture callus volume (P < 0.05) and biomechanical strength (P < 0.05) in animals with alcohol-impaired healing. In vivo imaging demonstrated a time-dependent MSC migration to the fracture site.
conclusionsThese data suggest that a 2-week binge alcohol exposure significantly impairs fracture healing in a murine tibia fracture model. Intravenously administered MSC were capable of specifically homing to the fracture site and of normalizing biomechanical, histologic, and microcomputed tomography parameters of healing in animals exposed to alcohol. Understanding MSC recruitment patterns and functional contributions to fracture repair may lead to their use in patients with impaired fracture healing and nonunion.
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