ArticleAnalytical chemistry2012
Laserspray ionization imaging of multiply charged ions using a commercial vacuum MALDI ion source.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- New Processes for Ionizing Nonvolatile Compounds in Mass Spectrometry: The Road of Discovery to Current State-of-the-Art.Journal of the American Society for Mass Spectrometry · 2024Review
- Direct sub-atmospheric pressure ionization mass spectrometry: Evaporation/sublimation-driven ionization is amazing, fundamentally, and practically.Journal of mass spectrometry : JMS · 2024Review
- Instrumentation development, improvement, simplification, and miniaturization: The multifunctional plate source for use in mass spectrometry.European journal of mass spectrometry (Chichester, England) · 2023Article
- Recent advances in mass spectrometry analysis of neuropeptides.Mass spectrometry reviews · 2023Review
- Multiomics Imaging Using High-Energy Water Gas Cluster Ion Beam Secondary Ion Mass Spectrometry [(HAnalytical chemistry · 2021Article
- Fundamental Studies of New Ionization Technologies and Insights from IMS-MS.Journal of the American Society for Mass Spectrometry · 2019Article
- A high resolution atmospheric pressure matrix-assisted laser desorption/ionization-quadrupole-orbitrap MS platform enables in situ analysis of biomolecules by multi-mode ionization and acquisition.Analytica chimica acta · 2018Article
- "Magic" Ionization Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2016Article
- Transmission geometry laserspray ionization vacuum using an atmospheric pressure inlet.Analytical chemistry · 2014Article
- High-speed tandem mass spectrometric in situ imaging by nanospray desorption electrospray ionization mass spectrometry.Analytical chemistry · 2013Article
- Liquid AP-UV-MALDI enables stable ion yields of multiply charged peptide and protein ions for sensitive analysis by mass spectrometry.Angewandte Chemie (International ed. in English) · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
This is the first report of imaging mass spectrometry (MS) from multiply charged ions at vacuum. Laserspray ionization (LSI) was recently extended to applications at vacuum producing electrospray ionization-like multiply charged ions directly from surfaces using a commercial intermediate pressure matrix-assisted laser desorption/ionization ion mobility spectrometry (IMS) MS instrument. Here, we developed a strategy to image multiply charged peptide ions. This is achieved by the use of 2-nitrophloroglucinol as matrix for spray deposition onto the tissue section and implementation of "soft" acquisition conditions including lower laser power and ion accelerating voltages similar to electrospray ionization-like conditions. Sufficient ion abundance is generated by the vacuum LSI method to employ IMS separation in imaging multiply charged ions obtained on a commercial mass spectrometer ion source without physical instrument modifications using the laser in the commercially available reflection geometry alignment. IMS gas-phase separation reduces the complexity of the ion signal from the tissue, especially for multiply charged relative to abundant singly charged ions from tissue lipids. We show examples of LSI tissue imaging from charge state +2 of three endogenous peptides consisting of between 1 and 16 amino acid residues from the acetylated N-terminal end of myelin basic protein: mass-to-charge (m/z) 795.81 (+2) molecular weight (MW) 1589.6, m/z 831.35 (+2) MW 1660.7, and m/z 917.40 (+2) MW 1832.8.
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