Evidence map›Paper›PMID 23009595›Full record

ArticleCurrent eye research2013

Na(+)/K(+)-ATPase expression changes in the rabbit lacrimal glands during pregnancy.

Jianyan Huang, Michael Lu, Chuanqing Ding

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Current eye research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Ion channels in dry eye disease.Indian journal of ophthalmology · 2023
    Review
  3. Should I Get LASIK If I'm Breastfeeding?Ophthalmology and therapy · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jianyan HuangDepartment of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089-9112, USA.
Michael Lu
Chuanqing Ding
Schlumberger (Ireland) · IE

Funding

VISION RESEARCHP30EY003040 · NEI · DOHENY EYE INSTITUTE · PI SUN, DEMING · 1985 to 2013
$7.0M
Functional Regulation of the Lacrimal Duct SystemR01EY017731 · NEI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI DING, CHUANQING · 2008 to 2011
$1.6M
NEI NIH HHS EY017731NEI NIH HHS EY03040NEI NIH HHS P30 EY003040NEI NIH HHS R01 EY017731
6 · The paper itself

Abstract

purposeTo investigate the expressional changes of Na(+)/K(+)-ATPase subunits in the lacrimal glands (LG) of term pregnant rabbits.

methodsLG were obtained from term pregnant rabbits and age-matched female control rabbits for laser capture microdissection (LCM), real time RT-PCR, western blot, and immunofluorescence. The mRNA and proteins of α1, α2, β1, β2, and β3 subunits of Na(+)/K(+)-ATPase were detected and quantified.

resultsAlthough only the mRNA for β3 from whole LG of pregnant rabbits was significantly different from that of normal controls, many mRNA levels for α1, α2, β1, β2, and β3 from acini and epithelial cells from various duct segments that were collected by LCM were significantly different from those of normal control rabbits. Western blots demonstrated that the expressions of all three β subunits were significantly higher in pregnant rabbits, while both α subunits remained unchanged during pregnancy. Interestingly, immunofluorescence results showed that the distribution patterns of all Na(+)/K(+)-ATPase subunits during pregnancy were similar to those of the control rabbits.

conclusionsChanges were found in mRNA and protein expressions of Na(+)/K(+)-ATPase subunits in LG from term pregnant rabbits and these changes suggest a role in the pregnancy-related LG secretion changes and dry eye symptoms observed in these animals.

Indexed as

Gene Expression RegulationPregnancy, AnimalAnimalsBlotting, WesternDisease Models, AnimalDry Eye SyndromesEpithelial CellsFemaleImmunohistochemistryLacrimal ApparatusLaser Capture MicrodissectionPregnancyRabbitsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSodium-Potassium-Exchanging ATPaseRNA, MessengerSodium-Potassium-Exchanging ATPase

Identifiers

PMID23009595
PMCPMC3636992
OpenAlexW2004785898

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.