ArticleBMC infectious diseases2012
Performance comparison of phenotypic and molecular methods for detection and differentiation of Candida albicans and Candida dubliniensis.
Article in BMC infectious diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Nucleic Acid Amplification Tests forPathogens (Basel, Switzerland) · 2026Pooled it
- Evaluation of Etest and MICRONAUT-AM Assay for Antifungal Susceptibility Testing ofAntibiotics (Basel, Switzerland) · 2024Article
- Oral candidiasis in liver transplant patients: species identification and antifungal susceptibility profile.Einstein (Sao Paulo, Brazil) · 2024Article
- Cutaneous candidiasis mimicking acute generalized exanthematous pustulosis: A case report.SAGE open medical case reports · 2023Article
- FatalJournal of fungi (Basel, Switzerland) · 2022Article
- The evaluation of the overexpression of the ERG-11, MDR-1, CDR-1, and CDR-2 genes in fluconazole-resistant Candida albicans isolated from Ahvazian cancer patients with oral candidiasis.Journal of clinical laboratory analysis · 2022Article
- Vulvovaginal candidiasis and current perspectives: new risk factors and laboratory diagnosis by using MALDI TOF for identifying species in primary infection and recurrence.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- First Isolation of Candida nivariensis, an Emerging Fungal Pathogen, in Kuwait.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2021Article
- Transcription Factors of CAT1, EFG1, and BCR1 Are Effective in Persister Cells ofFrontiers in microbiology · 2021Article
- Molecular Epidemiology ofJournal of fungi (Basel, Switzerland) · 2020Article
- Low prevalence of antifungal resistantHeliyon · 2020Article
- Candida kefyr in Kuwait: Prevalence, antifungal drug susceptibility and genotypic heterogeneity.PloS one · 2020Article
- An alarming rise of non-albicans Candida species and uncommon yeasts in the clinical samples; a combination of various molecular techniques for identification of etiologic agents.BMC research notes · 2019Article
- Successful Sequential Treatment with Itraconazole and ALA-PDT for Cutaneous Granuloma by Candida albicans: A Case Report and Literature Review.Mycopathologia · 2018Review
- Molecular characterization ofCurrent medical mycology · 2018Article
- Rapid and Accurate Identification of Candida albicans and Candida dubliniensis by Real-Time PCR and Melting Curve Analysis.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2018Article
- Identification ofAdvanced biomedical research · 2018Article
- Comparison of duplex PCR and phenotypic analysis in differentiating Candida dubliniensis from Candida albicans from oral samples.AMB Express · 2017Article
- A new assay based on terminal restriction fragment length polymorphism of homocitrate synthase gene fragments for Candida species identification.Journal of applied genetics · 2017Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundCandida albicans is the most pathogenic Candida species but shares many phenotypic features with Candida dubliniensis and may, therefore, be misidentified in clinical microbiology laboratories. Candidemia cases due to C. dubliniensis are increasingly being reported in recent years. Accurate identification is warranted since mortality rates are highest for C. albicans infections, however, C. dubliniensis has the propensity to develop resistance against azoles more easily. We developed a duplex PCR assay for rapid detection and differentiation of C. albicans from C. dubliniensis for resource-poor settings equipped with basic PCR technology and compared its performance with three phenotypic methods.
methodsDuplex PCR was performed on 122 germ tube positive and 12 germ tube negative isolates of Candida species previously identified by assimilation profiles on Vitek 2 ID-YST system. Typical morphologic characteristics on simplified sunflower seed agar (SSA), and reaction with a commercial (Bichro-Dubli) latex agglutination test were also performed. The assay was further applied on 239 clinical yeast and yeast-like fungi and results were confirmed by DNA sequencing of internal transcribed spacer (ITS) region of rDNA.
resultsThe results of duplex PCR assay for 122 germ tube positive and 12 germ tube negative isolates of Candida species were comparable to their identification by Vitek 2 ID-YST system, colony characteristics on SSA and latex agglutination test. Application of duplex PCR also correctly identified all 148 C. albicans and 50 C. dubliniensis strains among 239 yeast-like fungi.
conclusionsThe data show that both, duplex PCR and Bichro-Dubli are reliable tests for rapid (within few hours) identification of clinical yeast isolates as C. dubliniensis or C. albicans. However, duplex PCR may be applied directly on clinical yeast isolates for their identification as C. dubliniensis or C. albicans as it does not require prior testing for germ tube formation or latex Candida agglutination.
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