ArticlePlanta2013
Characterization of the stress-inducible OsNCED3 promoter in different transgenic rice organs and over three homozygous generations.
Article in Planta, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- An ABA biosynthesis enzyme gene OsNCED4 regulates NaCl and cold stress tolerance in rice.Scientific reports · 2024Article
- Overexpression of Abscisic Acid Biosynthesis GenePlants (Basel, Switzerland) · 2024Article
- Strigolactone and abscisic acid synthesis and signaling pathways are enhanced in the wheat oligo-tillering mutantMolecular breeding : new strategies in plant improvement · 2024Article
- Characterization of theInternational journal of molecular sciences · 2023Article
- Uncovering the role of wheat magnesium transporter family genes in abiotic responses.Frontiers in plant science · 2023Article
- The root-specific NtR12 promoter-based expression of RIP increased the resistance against bacterial wilt disease in tobacco.Molecular biology reports · 2022Article
- The miR528-D3 Module Regulates Plant Height in Rice by Modulating the Gibberellin and Abscisic Acid Metabolisms.Rice (New York, N.Y.) · 2022Article
- TaASR1-D confers abiotic stress resistance by affecting ROS accumulation and ABA signalling in transgenic wheat.Plant biotechnology journal · 2021Article
- Expression Analyses of Soybean VOZ Transcription Factors and the Role ofInternational journal of molecular sciences · 2020Article
- An update and perspectives on the use of promoters in plant genetic engineering.Journal of biosciences · 2020Review
- Genome-Wide Identification of SNAC1-Targeted Genes Involved in Drought Response in Rice.Frontiers in plant science · 2019Article
- Synthetic Promoters: Designing the cis Regulatory Modules for Controlled Gene Expression.Molecular biotechnology · 2018Review
- Heterologous expression of rice 9-cis-epoxycarotenoid dioxygenase 4 (OsNCED4) in Arabidopsis confers sugar oversensitivity and drought tolerance.Botanical studies · 2018Article
- 9-Frontiers in plant science · 2018Article
- Surviving a Dry Future: Abscisic Acid (ABA)-Mediated Plant Mechanisms for Conserving Water under Low Humidity.Plants (Basel, Switzerland) · 2017Review
- Transcription Factors and Plants Response to Drought Stress: Current Understanding and Future Directions.Frontiers in plant science · 2016Review
- Article
- Article
- A distal ABA responsive element in AtNCED3 promoter is required for positive feedback regulation of ABA biosynthesis in Arabidopsis.PloS one · 2014Article
- Comparative functional analysis of six drought-responsive promoters in transgenic rice.Planta · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To be effective in crop biotechnology applications, gene promoters need to be stably active over sequential generations in a population of single-copy transgenic lines. Most of the stress-inducible promoters characterized in plants thus far have been analyzed at early (T₀, T₁ or T₂) generations and/or by testing only a small number of transgenic lines. In our current study, we report our analysis of OsNCED3, a stress-inducible rice promoter involved in ABA biosynthesis, in various organs and tissues of transgenic rice plants over the T(2-4) homozygous generations. The transgene copy numbers in the lines harboring the OsNCED3:gfp construct were determined and six single- and two double-copy transgenic lines were analyzed for promoter activity in comparison with the Wsi18, a stress-inducible promoter previously characterized. The exogenous promoter activities were found to be significantly enhanced in the roots and leaves, whereas zero or low levels of activity were evident in grains and flowers, under drought and high-salinity conditions. The highest induction levels of gfp transcripts in the OsNCED3:gfp plants upon drought treatments were 161- and 93-fold in leaves and roots, respectively, and these levels were comparable with those of gfp transcripts in the Wsi18:gfp plants. A comparison of the promoter activities between the T₂-T₄ plants revealed that comparable activity levels were maintained over these three homozygous generations with no evidence of silencing. Thus, our results provide the OsNCED3 promoter that is stress-inducible in a whole rice plant except for in the aleurones and endosperm and stably active over three generations.
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